ArticleNature methods2010
Floxin, a resource for genetically engineering mouse ESCs.
Article in Nature methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
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- A most formidable arsenal: genetic technologies for building a better mouse.Genes & development · 2020Review
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- Quantitative Histone Mass Spectrometry Identifies Elevated Histone H3 Lysine 27 (Lys27) Trimethylation in Melanoma.Molecular & cellular proteomics : MCP · 2016Article
- Knockin of Cre Gene at Ins2 Locus Reveals No Cre Activity in Mouse Hypothalamic Neurons.Scientific reports · 2016Article
- Two-Pore Channels: Lessons from Mutant Mouse Models.Messenger (Los Angeles, Calif. : Print) · 2015Article
- Chd1 is essential for the high transcriptional output and rapid growth of the mouse epiblast.Development (Cambridge, England) · 2015Article
- A central region of Gli2 regulates its localization to the primary cilium and transcriptional activity.Journal of cell science · 2014Article
- Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal.PLoS genetics · 2012Article
- The ciliogenic protein Oral-Facial-Digital 1 regulates the neuronal differentiation of embryonic stem cells.Stem cells and development · 2011Article
- Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.Nucleic acids research · 2010Article
- Dual RMCE for efficient re-engineering of mouse mutant alleles.Nature methods · 2010Article
- Ofd1, a human disease gene, regulates the length and distal structure of centrioles.Developmental cell · 2010Article
- Polycomb-like 2 associates with PRC2 and regulates transcriptional networks during mouse embryonic stem cell self-renewal and differentiation.Cell stem cell · 2010Article
- Gene trap mutagenesis in the mouse.Methods in enzymology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.