ArticleProceedings of the National Academy of Sciences of the United States of America2010
DNA methylation of developmental genes in pediatric medulloblastomas identified by denaturation analysis of methylation differences.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 63 citations in OpenAlex.
- Extracellular ssDNA fromInternational journal of molecular sciences · 2026Article
- The transcription factor LHX2 mediates and enhances oncogenic BMP signaling in medulloblastoma.Cell death and differentiation · 2025Article
- Role of epigenetics in paediatric cancer pathogenesis & drug resistance.British journal of cancer · 2025Review
- A Practical Approach for Targeting Structural Variants Genome-wide in Plasma Cell-free DNA.Research square · 2024Article
- Genome-Wide Analysis of Palindrome Formation with Next-Generation Sequencing (GAPF-Seq) and a Bioinformatics Pipeline for Assessing De Novo Palindromes in Cancer Genomes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Epigenetic regulation in medulloblastoma.Molecular and cellular neurosciences · 2018Review
- The Hedgehog-GLI pathway in embryonic development and cancer: implications for pulmonary oncology therapy.Oncotarget · 2017Review
- Palindromic amplification of the ERBB2 oncogene in primary HER2-positive breast tumors.Scientific reports · 2017Article
- The evolution of medulloblastoma therapy to personalized medicine.F1000Research · 2017Review
- Sonic Hedgehog Signaling Affected by Promoter Hypermethylation Induces Aberrant Gli2 Expression in Spina Bifida.Molecular neurobiology · 2016Article
- Non-DHFR-mediated effects of methotrexate in osteosarcoma cell lines: epigenetic alterations and enhanced cell differentiation.Cancer cell international · 2016Article
- Effects of non-CpG site methylation on DNA thermal stability: a fluorescence study.Nucleic acids research · 2015Article
- Overexpression of mutant Ptch in rhabdomyosarcomas is associated with promoter hypomethylation and increased Gli1 and H3K4me3 occupancy.Oncotarget · 2015Article
- Epigenetic therapy of cancer stem and progenitor cells by targeting DNA methylation machineries.World journal of stem cells · 2015Review
- Deregulated proliferation and differentiation in brain tumors.Cell and tissue research · 2015Review
- Article
- Fundamental differences in promoter CpG island DNA hypermethylation between human cancer and genetically engineered mouse models of cancer.Epigenetics · 2013Article
- Homology-mediated end-capping as a primary step of sister chromatid fusion in the breakage-fusion-bridge cycles.Nucleic acids research · 2013Article
- Identification of vitamin d3-based hedgehog pathway inhibitors that incorporate an aromatic a-ring isostere.ACS medicinal chemistry letters · 2013Article
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8 authors at 4 institutions in 1 country.
Funding
Abstract
DNA methylation might have a significant role in preventing normal differentiation in pediatric cancers. We used a genomewide method for detecting regions of CpG methylation on the basis of the increased melting temperature of methylated DNA, termed denaturation analysis of methylation differences (DAMD). Using the DAMD assay, we find common regions of cancer-specific methylation changes in primary medulloblastomas in critical developmental regulatory pathways, including Sonic hedgehog (Shh), Wingless (Wnt), retinoic acid receptor (RAR), and bone morphogenetic protein (BMP). One of the commonly methylated loci is the PTCH1-1C promoter, a negative regulator of the Shh pathway that is methylated in both primary patient samples and human medulloblastoma cell lines. Treatment with the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine (5-aza-dC) increases the expression of PTCH1 and other methylated loci. Whereas genetic mutations in PTCH1 have previously been shown to lead to medulloblastoma, our study indicates that epigenetic silencing of PTCH1, and other critical developmental loci, by DNA methylation is a fundamental process of pediatric medulloblastoma formation. This finding warrants strong consideration for DNA demethylating agents in future clinical trials for children with this disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.