ArticleEndocrinology2010
Vitamin D metabolism and action in human bone marrow stromal cells.
Article in Endocrinology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 91 citations in OpenAlex.
- The effect of high-dose vitamin DOsteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2018Trial
- Review
- A threshold of β-CTX (0.3 ng/mL) with low estradiol identifies high-risk perimenopausal women for bone loss: a cross-sectional study.Frontiers in endocrinology · 2025Article
- Fat as a Friend or Foe of the Bone.Current osteoporosis reports · 2024Review
- Review
- 25-hydroxyvitamin DFrontiers in immunology · 2023Article
- Article
- Effects of Parenteral Vitamin D3 Supplementation on Hematological Parameters of Healthy Holstein Bulls.Archives of Razi Institute · 2021Article
- Recommendations on the measurement and the clinical use of vitamin D metabolites and vitamin D binding protein - A position paper from the IFCC Committee on bone metabolism.Clinica chimica acta; international journal of clinical chemistry · 2021Review
- Identification of a clinical signature predictive of differentiation fate of human bone marrow stromal cells.Stem cell research & therapy · 2021Article
- The Crossroads between Infection and Bone Loss.Microorganisms · 2020Review
- The Regulation of Marrow Fat by Vitamin D: Molecular Mechanisms and Clinical Implications.Current osteoporosis reports · 2019Review
- Vitamin D Metabolism Revised: Fall of Dogmas.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Review
- Physiologic and pathophysiologic roles of extra renal CYP27b1: Case report and review.Bone reports · 2018Article
- Chronic kidney disease and vitamin D metabolism in human bone marrow-derived MSCs.Annals of the New York Academy of Sciences · 2017Review
- 25-Hydroxyvitamin DScientific reports · 2017Article
- Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice.BioMed research international · 2017Article
- Zoledronic acid in vivo increases in vitro proliferation of rat mesenchymal stromal cells.Acta orthopaedica · 2016Article
- Transplantation of bone marrow-derived mesenchymal stem cells rescues partially rachitic phenotypes induced by 1,25-Dihydroxyvitamin D deficiency in mice.American journal of translational research · 2016Article
- Vitamin D Effects on Osteoblastic Differentiation of Mesenchymal Stem Cells from Dental Tissues.Stem cells international · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Vitamin D metabolites are important effectors of bone and mineral homeostasis. Extrarenal conversion of 25-hydroxyvitamin D (25OHD) to the biologically active form of vitamin D, 1 alpha,25-dihydroxyvitamin D [1,25(OH)(2)D] is catalyzed in several cell types by the 1 alpha-hydroxylase (CYP27B1), but little is known about the expression or regulation of CYP27B1 in human bones. We examined whether human bone marrow stromal cells (hMSCs, also known as mesenchymal stem cells) participate in vitamin D metabolism and whether vitamin D hydroxylases in hMSCs are influenced by the vitamin D status of the individual from whom the hMSCs were obtained. We also investigated the effects of vitamin D metabolites on osteoblast differentiation and the role of IGF-I in the regulation of CYP27B1. In a series of 27 subjects, vitamin D hydroxylases in hMSCs were expressed at different levels and were correlated with serum 25OHD, 1,25(OH)(2)D, and PTH. In vitro treatment with 25OHD up-regulated CYP27B1 and IGF-I in hMSCs; IGF-I also up-regulated CY27B1 expression and stimulated osteoblast differentiation. When hydroxylation of 25OHD was blocked by ketoconazole, a cytochrome P450 inhibitor, 25OHD was no longer able to induce CYP27B1 expression. In summary, these findings show that human bone marrow stromal cells have the molecular machinery both to metabolize and respond to vitamin D. We propose that circulating 25OHD, by virtue of its local conversion to 1,25(OH)(2)D catalyzed by basal CYP27B1 in hMSCs, amplifies vitamin D signaling through IGF-I up-regulation, which in turn induces CYP27B1 in a feed-forward mechanism to potentiate osteoblast differentiation initiated by IGF-I.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.