ArticleApplied and environmental microbiology2010
Use of rpsL as a Counterselectable Marker in Borrelia burgdorferi.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Development of a counterselection system for efficient marker-free genetic manipulation inApplied and environmental microbiology · 2026Article
- Genetic Manipulation ofCurrent issues in molecular biology · 2021Review
- Recent Advances in Genetic Tools forFrontiers in genetics · 2020Review
- Fluorescent Proteins, Promoters, and Selectable Markers for Applications in the Lyme Disease Spirochete Borrelia burgdorferi.Applied and environmental microbiology · 2018Article
- Genetic Transformation and Complementation.Methods in molecular biology (Clifton, N.J.) · 2018Article
- pyrF as a Counterselectable Marker for Unmarked Genetic Manipulations in Treponema denticola.Applied and environmental microbiology · 2016Article
- A suitable streptomycin-resistant mutant for constructing unmarked in-frame gene deletions using rpsL as a counter-selection marker.PloS one · 2014Article
- Illuminating the roles of the Borrelia burgdorferi adhesins.Trends in microbiology · 2013Review
- Reversing bacterial resistance to antibiotics by phage-mediated delivery of dominant sensitive genes.Applied and environmental microbiology · 2012Article
- Genetics of Borrelia burgdorferi.Annual review of genetics · 2012Review
- Genetic transformation of Veillonella parvula.FEMS microbiology letters · 2011Article
- Development and use of a gene deletion strategy for Flavobacterium johnsoniae to identify the redundant gliding motility genes remF, remG, remH, and remI.Journal of bacteriology · 2011Article
- Genetic Manipulation of Borrelia Spp.Current topics in microbiology and immunologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have demonstrated that rpsL, encoding the S12 protein of the small ribosomal subunit, can be used as a counterselectable marker in Borrelia burgdorferi, the causative agent of Lyme disease. Mutations in rpsL confer streptomycin resistance. Streptomycin susceptibility is dominant in an rpsL merodiploid, and streptomycin selects for the loss of wild-type rpsL carried in trans. This is the first description of a counterselectable marker in B. burgdorferi.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.