ArticleNucleic acids research2010
Precise targeted integration by a chimaeric transposase zinc-finger fusion protein.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Retargeted serine integrases for one-step, precise integration of large DNA sequences in human cells.Nature biotechnology · 2026Article
- Gene editing and its applications in biomedicine.Science China. Life sciences · 2022Review
- Potential of transposon-mediated cellular reprogramming towards cell-based therapies.World journal of stem cells · 2020Review
- RNA-guided retargeting of SeLife · 2020Article
- An Engineered Cas-Transposon System for Programmable and Site-Directed DNA Transpositions.The CRISPR journal · 2019Article
- Targeted DNA transposition in vitro using a dCas9-transposase fusion protein.Nucleic acids research · 2019Article
- A Mini-ISY100 Transposon Delivery System Effective in γ Proteobacteria.Frontiers in microbiology · 2019Article
- Engineering and Application of Zinc Finger Proteins and TALEs for Biomedical Research.Molecules and cells · 2017Review
- Article
- Transcription activator like effector (TALE)-directed piggyBac transposition in human cells.Nucleic acids research · 2013Article
- Article
- Retargeting sleeping beauty transposon insertions by engineered zinc finger DNA-binding domains.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- Retargeting transposon insertions by the adeno-associated virus Rep protein.Nucleic acids research · 2012Article
- Chimeric piggyBac transposases for genomic targeting in human cells.Nucleic acids research · 2012Article
- A novel zinc-finger nuclease platform with a sequence-specific cleavage module.Nucleic acids research · 2012Article
- Moving DNA around: DNA transposition and retroviral integration.Current opinion in structural biology · 2011Review
- Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.Current gene therapy · 2011Review
- Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology.Nucleic acids research · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Transposons of the Tc1/mariner family have been used to integrate foreign DNA stably into the genome of a large variety of different cell types and organisms. Integration is at TA dinucleotides located essentially at random throughout the genome, potentially leading to insertional mutagenesis, inappropriate activation of nearby genes, or poor expression of the transgene. Here, we show that fusion of the zinc-finger DNA-binding domain of Zif268 to the C-terminus of ISY100 transposase leads to highly specific integration into TA dinucleotides positioned 6-17 bp to one side of a Zif268 binding site. We show that the specificity of targeting can be changed using Zif268 variants that bind to sequences from the HIV-1 promoter, and demonstrate a bacterial genetic screen that can be used to select for increased levels of targeted transposition. A TA dinucleotide flanked by two Zif268 binding sites was efficiently targeted by our transposase-Zif268 fusion, suggesting the possibility of designer 'Z-transposases' that could deliver transgenic cargoes to chosen genomic locations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.