ArticleNucleic acids research2010
A calibrated diversity assay for nucleic acid libraries using DiStRO--a Diversity Standard of Random Oligonucleotides.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Integrating Magnetic Bead-Based SELEX withACS omega · 2025Article
- IMPATIENT-qPCR: monitoring SELEX success during in vitro aptamer evolution.Applied microbiology and biotechnology · 2024Article
- Selection of a novel cell-internalizing RNA aptamer specific for CD22 antigen in B cell acute lymphoblastic leukemia.Molecular therapy. Nucleic acids · 2023Article
- Therapeutic Interventions into Innate Immune Diseases by Means of Aptamers.Pharmaceutics · 2020Review
- Using a PCR-Based Method To Analyze and Model Large, Heterogeneous Populations of DNA.Chembiochem : a European journal of chemical biology · 2020Article
- Inside the Black Box: What Makes SELEX Better?Molecules (Basel, Switzerland) · 2019Review
- Anti-FGFR1 aptamer-tagged superparamagnetic conjugates for anticancer hyperthermia therapy.International journal of nanomedicine · 2017Article
- Real-Time PCR: an Appropriate Approach to Confirm ssDNA Generation from PCR Product in SELEX Process.Iranian journal of biotechnology · 2017Article
- New Technologies Provide Quantum Changes in the Scale, Speed, and Success of SELEX Methods and Aptamer Characterization.Molecular therapy. Nucleic acids · 2014Article
- A generalized mathematical model to estimate T- and B-cell receptor diversities using AmpliCot.Biophysical journal · 2012Article
- Rapid identification of cell-specific, internalizing RNA aptamers with bioinformatics analyses of a cell-based aptamer selection.PloS one · 2012Article
- Design, construction, and validation of a modular library of sequence diversity standards for polymerase chain reaction.Analytical biochemistry · 2011Article
- RNA aptamer evolution: two decades of SELEction.International journal of molecular sciences · 2011Review
- Probing the SELEX process with next-generation sequencing.PloS one · 2011Article
- Automation in the high-throughput selection of random combinatorial libraries--different approaches for select applications.Molecules (Basel, Switzerland) · 2010Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have determined diversities exceeding 10(12) different sequences in an annealing and melting assay using synthetic randomized oligonucleotides as a standard. For such high diversities, the annealing kinetics differ from those observed for low diversities, favouring the remelting curve after annealing as the best indicator of complexity. Direct comparisons of nucleic acid pools obtained from an aptamer selection demonstrate that even highly complex populations can be evaluated by using DiStRO, without the need of complicated calculations.
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Registered trials
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