ArticleProceedings of the National Academy of Sciences of the United States of America1991
Chromostatin, a 20-amino acid peptide derived from chromogranin A, inhibits chromaffin cell secretion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 140 citations in OpenAlex.
- Chromogranin A: An Endocrine Factor of Pregnancy.International journal of molecular sciences · 2023Review
- The plasminogen activation system and the regulation of catecholaminergic function.Journal of biomedicine & biotechnology · 2012Review
- Immunohistochemical and biochemical studies with region-specific antibodies to chromogranins A and B and secretogranins II and III in neuroendocrine tumors.Cellular and molecular neurobiology · 2010Review
- Mass spectrometry-based neuropeptidomics of secretory vesicles from human adrenal medullary pheochromocytoma reveals novel peptide products of prohormone processing.Journal of proteome research · 2010Article
- Beta cell chromogranin B is partially segregated in distinct granules and can be released separately from insulin in response to stimulation.Diabetologia · 2008Article
- Chromogranin A in gastric neuroendocrine tumours: an immunohistochemical and biochemical study with region-specific antibodies.Virchows Archiv : an international journal of pathology · 2006Article
- Chromogranin a processing in human pituitary adenomas and carcinomas: analysis with region-specific antibodies.Endocrine pathology · 2003Article
- Processing of chromogranin A by plasmin provides a novel mechanism for regulating catecholamine secretion.The Journal of clinical investigation · 2000Article
- The chromogranins and the counter-regulatory hormones: do they make homeostatic sense?The Journal of physiology · 1999Article
- Novel autocrine feedback control of catecholamine release. A discrete chromogranin a fragment is a noncompetitive nicotinic cholinergic antagonist.The Journal of clinical investigation · 1997Article
- Pancreastatin secretion by pituitary adenomas and regulation of chromogranin B mRNA expression.The American journal of pathology · 1996Article
- Analysis of the chromogranin A post-translational cleavage product pancreastatin and the prohormone convertases PC2 and PC3 in normal and neoplastic human pituitaries.The American journal of pathology · 1995Article
- Immunohistochemical Localization of Chromostatin and Pancreastatin, Chromogranin A-Derived Bioactive Peptides, in Normal and Neoplastic Neuroendocrine Tissues.Endocrine pathology · 1995Article
- The post-translational processing of chromogranin A in the pancreatic islet: involvement of the eukaryote subtilisin PC2.The Biochemical journal · 1994Article
- The granin protein family: markers for neuroendocrine cells and tools for the diagnosis of neuroendocrine tumors.Journal of endocrinological investigation · 1994Review
- Expression of chromogranin A and B and secretoneurin immunoreactivity in neoplastic and nonneoplastic pancreatic alpha cells.Virchows Archiv : an international journal of pathology · 1994Article
- The adrenal chromaffin granule: a model for large dense core vesicles of endocrine and nervous tissue.Journal of anatomy · 1993Review
- Novel peptides from adrenomedullary chromaffin vesicles.Journal of anatomy · 1993Article
- Secretory protein traffic. Chromogranin A contains a dominant targeting signal for the regulated pathway.The Journal of clinical investigation · 1993Article
- Chromostatin, a chromogranin A-derived bioactive peptide, is present in human pancreatic insulin (beta) cells.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromogranin A (CGA) is a ubiquitous 48-kDa secretory protein present in adrenal medulla, anterior pituitary, central and peripheral nervous system, endocrine gut, thyroid, parathyroid, and endocrine pancreas. Recently, we have demonstrated that the protein could be a precursor of bioactive peptides capable of modulating catecholamine secretion from cultured adrenal medullary chromaffin cells. Here we cleaved CGA purified from bovine chromaffin granules with endoproteinase Lys-C, and we isolated and partially sequenced the peptide inhibiting catecholamine secretion from cultured chromaffin cells. A corresponding synthetic peptide composed of the first 20 N-terminal amino acids produced a dose-dependent inhibition in the 10(-9) to 10(-6) M range (with an ID50 of 5 nM) of the catecholamine secretion evoked by carbamoylcholine or by potassium at a depolarizing concentration. This peptide affected secretagogue-induced calcium fluxes but did not alter sodium fluxes. It was found to increase desensitization of cell responses and to modify the kinetics of catecholamine release. Our results indicate that the peptide is extracellularly generated from CGA by a calcium-dependent proteolytic mechanism. We suggest that this peptide, named chromostatin, may be an endocrine modulator of catecholamine-associated responses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.