ArticleCurrent biology : CB2009
The F-BAR protein Syp1 negatively regulates WASp-Arp2/3 complex activity during endocytic patch formation.
Article in Current biology : CB, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed, 68 citations in OpenAlex.
- Sequential recruitment of F-BAR proteins controls cytoskeletal crosstalk at the yeast bud neck.Current biology : CB · 2025Article
- Spatio-temporal regulation of endocytic protein assembly by SH3 domains in yeast.Molecular biology of the cell · 2023Article
- Regulation of membrane scission in yeast endocytosis.Molecular biology of the cell · 2022Article
- The Role of the Cell Integrity Pathway in Septum Assembly in Yeast.Journal of fungi (Basel, Switzerland) · 2021Review
- Identification of Suppressor of Clathrin Deficiency-1 (G3 (Bethesda, Md.) · 2019Article
- The Sla1 adaptor-clathrin interaction regulates coat formation and progression of endocytosis.Traffic (Copenhagen, Denmark) · 2018Article
- A Flow Cytometry-Based Phenotypic Screen To Identify Novel Endocytic Factors inG3 (Bethesda, Md.) · 2018Article
- The TORC2-Dependent Signaling Network in the Yeast Saccharomyces cerevisiae.Biomolecules · 2017Review
- Syp1 regulates the clathrin-mediated and clathrin-independent endocytosis of multiple cargo proteins through a novel sorting motif.Molecular biology of the cell · 2017Article
- Linking up at the BAR: Oligomerization and F-BAR protein function.Cell cycle (Georgetown, Tex.) · 2016Review
- Polarized Exocytosis Induces Compensatory Endocytosis by Sec4p-Regulated Cortical Actin Polymerization.PLoS biology · 2016Article
- Identification of an ATP-controlled allosteric switch that controls actin filament nucleation by Arp2/3 complex.Nature communications · 2016Article
- Creating a chimeric clathrin heavy chain that functions independently of yeast clathrin light chain.Traffic (Copenhagen, Denmark) · 2016Article
- Clathrin-mediated endocytosis in budding yeast at a glance.Journal of cell science · 2016Review
- Septin-Associated Protein Kinases in the YeastFrontiers in cell and developmental biology · 2016Review
- Article
- Rho1- and Pkc1-dependent phosphorylation of the F-BAR protein Syp1 contributes to septin ring assembly.Molecular biology of the cell · 2015Article
- Cytoskeletal dynamics: a view from the membrane.The Journal of cell biology · 2015Review
- PICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner.Molecular biology of the cell · 2015Article
- A second Las17 monomeric actin-binding motif functions in Arp2/3-dependent actin polymerization during endocytosis.Traffic (Copenhagen, Denmark) · 2015Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundActin polymerization by Arp2/3 complex must be tightly regulated to promote clathrin-mediated endocytosis. Although many Arp2/3 complex activators have been identified, mechanisms for its negative regulation have remained more elusive. To address this, we analyzed the yeast arp2-7 allele, which is biochemically unique in causing unregulated actin assembly in vitro in the absence of Arp2/3 activators.
resultsWe examined endocytosis in arp2-7 mutants by live-cell imaging of Sla1-GFP, a coat marker, and Abp1-RFP, which marks the later actin phase of endocytosis. Sla1-GFP and Abp1-RFP lifetimes were accelerated in arp2-7 mutants, which is opposite to actin nucleation-impaired arp2 alleles or deletions of Arp2/3 activators. We performed a screen for multicopy suppressors of arp2-7 and identified SYP1, an FCHO1 homolog, which contains F-BAR and AP-2micro homology domains. Overexpression of SYP1 in arp2-7 cells slowed Sla1-GFP lifetimes closer to wild-type cells. Further, purified Syp1 directly inhibited Las17/WASp stimulation of Arp2/3 complex-mediated actin assembly in vitro. This activity was mapped to a fragment of Syp1 located between its F-BAR and AP-2micro homology domains and depends on sequences in Las17/WASp outside of the VCA domain.
conclusionsTogether, these data identify Syp1 as a novel negative regulator of WASp-Arp2/3 complex that helps choreograph the precise timing of actin assembly during endocytosis.
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