ArticleCancer science2010
Imaging mass spectrometry of gastric carcinoma in formalin-fixed paraffin-embedded tissue microarray.
Article in Cancer science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 95 citations in OpenAlex.
- Identification of Gene Signatures Differentiating Cancer from Normal Tissues Across Histological Classifications of Gastric Adenocarcinoma via Machine Learning Methods.Biochemical genetics · 2026Article
- Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry.Nature reviews. Methods primers · 2026Article
- Emerging applications of metabolomics in food science and future trends.Food chemistry: X · 2022Article
- DiviK: divisive intelligent K-means for hands-free unsupervised clustering in big biological data.BMC bioinformatics · 2022Article
- Techniques for Profiling the Cellular Immune Response and Their Implications for Interventional Oncology.Cancers · 2022Review
- Cell-blocks and other ancillary studies (including molecular genetic tests and proteomics).CytoJournal · 2021Review
- Stearate-to-palmitate ratio modulates endoplasmic reticulum stress and cell apoptosis in non-B non-C hepatoma cells.Cancer science · 2018Article
- Matrix-Assisted Laser Desorption/Ionisation Mass Spectrometry Imaging in the Study of Gastric Cancer: A Mini Review.International journal of molecular sciences · 2017Review
- Clinical significance of PD-L1 and PD-L2 copy number gains in non-small-cell lung cancer.Oncotarget · 2016Article
- Visualization of sphingolipids and phospholipids in the fundic gland mucosa of human stomach using imaging mass spectrometry.World journal of gastrointestinal pathophysiology · 2016Article
- Two-dimensional graphene as a matrix for MALDI imaging mass spectrometry.Journal of the American Society for Mass Spectrometry · 2015Article
- MALDI Imaging mass spectrometry: current frontiers and perspectives in pathology research and practice.Laboratory investigation; a journal of technical methods and pathology · 2015Review
- Applications of mass spectrometry for quantitative protein analysis in formalin-fixed paraffin-embedded tissues.Proteomics · 2014Review
- Current opportunities and challenges of magnetic resonance spectroscopy, positron emission tomography, and mass spectrometry imaging for mapping cancer metabolism in vivo.BioMed research international · 2014Review
- Analysis of protein tyrosine phosphatase interactions with microarrayed phosphopeptide substrates using imaging mass spectrometry.Analytical biochemistry · 2013Article
- Accumulated phosphatidylcholine (16:0/16:1) in human colorectal cancer; possible involvement of LPCAT4.Cancer science · 2013Article
- Article
- Recent advances in single-cell MALDI mass spectrometry imaging and potential clinical impact.Expert review of proteomics · 2011Review
- MALDI imaging mass spectrometry for direct tissue analysis: technological advancements and recent applications.Histochemistry and cell biology · 2011Review
- Imaging of intact tissue sections: moving beyond the microscope.The Journal of biological chemistry · 2011Review
Corrections and comments
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The popularity of imaging mass spectrometry (IMS) of tissue samples, which enables the direct scanning of tissue sections within a short time-period, has been considerably increasing in cancer proteomics. Most pathological specimens stored in medical institutes are formalin-fixed; thus, they had been regarded to be unsuitable for proteomic analyses, including IMS, until recently. Here, we report an easy-to-use screening method that enables the analysis of multiple samples in one experiment without extractions and purifications of proteins. We scanned, with an IMS technique, a tissue microarray (TMA) of formalin-fixed paraffin-embedded (FFPE) specimens. We detected a large amount of signals from trypsin-treated FFPE-TMA samples of gastric carcinoma tissues of different histological types. Of the signals detected, 54 were classified as signals specific to cancer with statistically significant differences between adenocarcinomas and normal tissues. We detected a total of 14 of the 54 signals as histological type-specific with the support of statistical analyses. Tandem MS revealed that a signal specific to poorly differentiated cancer tissue corresponded to histone H4. Finally, we verified the IMS-based finding by immunohistochemical analysis of more than 300 specimens spotted on TMAs; the immunoreactivity of histone H4 was remarkably strong in poorly differentiated cancer tissues. Thus, the application of IMS to FFPE-TMA can enable high-throughput analysis in cancer proteomics to aid in the understanding of molecular mechanisms underlying carcinogenesis, invasiveness, metastasis, and prognosis. Further, results obtained from the IMS of FFPE-TMA can be readily confirmed by commonly used immunohistochemical analyses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.