ArticleLangmuir : the ACS journal of surfaces and colloids2010
Orientation difference of chemically immobilized and physically adsorbed biological molecules on polymers detected at the solid/liquid interfaces in situ.
Article in Langmuir : the ACS journal of surfaces and colloids, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 66 citations in OpenAlex.
- Antimicrobial Peptides as Probes in Biosensors Detecting Whole Bacteria: A Review.Molecules (Basel, Switzerland) · 2020Review
- Nonlinear Optical Methods for Characterization of Molecular Structure and Surface Chemistry.Topics in catalysis · 2018Article
- Site-specific orientation of an α-helical peptide ovispirin-1 from isotope-labeled SFG spectroscopy.The journal of physical chemistry. B · 2013Article
- Membrane orientation of Gα(i)β(1)γ(2) and Gβ(1)γ(2) determined via combined vibrational spectroscopic studies.Journal of the American Chemical Society · 2013Article
- Protein-phospholipid interactions in nonclassical protein secretion: problem and methods of study.International journal of molecular sciences · 2013Article
- Elucidation of molecular structures at buried polymer interfaces and biological interfaces using sum frequency generation vibrational spectroscopy.Soft matter · 2013Article
- Molecular interactions of proteins and peptides at interfaces studied by sum frequency generation vibrational spectroscopy.Langmuir : the ACS journal of surfaces and colloids · 2012Review
- Effect of substrate (ZnO) morphology on enzyme immobilization and its catalytic activity.Nanoscale research letters · 2011Article
- Solvent effect and time-dependent behavior of C-terminus-cysteine-modified cecropin P1 chemically immobilized on a polymer surface.Langmuir : the ACS journal of surfaces and colloids · 2011Article
- Single Lipid Bilayers Constructed on Polymer Cushion Studied by Sum Frequency Generation Vibrational Spectroscopy.The journal of physical chemistry. C, Nanomaterials and interfaces · 2011Article
- Interactions of alamethicin with model cell membranes investigated using sum frequency generation vibrational spectroscopy in real time in situ.The journal of physical chemistry. B · 2010Article
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Authors and funding
5 authors at 2 institutions in 1 country.
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Abstract
A surface sensitive second order nonlinear optical technique, sum frequency generation vibrational spectroscopy, was applied to study peptide orientation on polymer surfaces, supplemented by a linear vibrational spectroscopy, attenuated total reflectance Fourier transform infrared spectroscopy. Using the antimicrobial peptide Cecropin P1 as a model system, we have quantitatively demonstrated that chemically immobilized peptides on polymers adopt a more ordered orientation than less tightly bound physically adsorbed peptides. These differences were also observed in different chemical environments, for example, air versus water. Although numerous studies have reported a direct correlation between the choice of immobilization method and the performance of an attached biological molecule, the lack of direct biomolecular structure and orientation data has made it difficult to elucidate the relationship between structure, orientation, and function at a surface. In this work, we directly studied the effect of chemical immobilization method on biomolecular orientation/ordering, an important step for future studies of biomolecular activity. The methods for orientation analysis described within are also of relevance to understanding biosensors, biocompatibility, marine-antifouling, membrane protein functions, and antimicrobial peptide activities.
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