ArticleMolecular and cellular biology1991
Tissue-specific transcription of the cardiac myosin light-chain 2 gene is regulated by an upstream repressor element.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 49 citations in OpenAlex.
- Expression of myotilin during chicken development.Anatomical record (Hoboken, N.J. : 2007) · 2014Article
- Repression of the cardiac myosin light chain-2 gene in skeletal muscle requires site-specific association of antithetic regulator, Nished, and HDACs.Journal of cellular and molecular medicine · 2009Article
- Requirement of TCTG(G/C) Direct Repeats and Overlapping GATA Site for Maintaining the Cardiac-Specific Expression of Cardiac troponin T in Developing and Adult Mice.Anatomical record (Hoboken, N.J. : 2007) · 2008Article
- Modulation of MLC-2v gene expression by AP-1: complex regulatory role of Jun in cardiac myocytes.Molecular and cellular biochemistry · 2001Article
- Tissue-restricted expression of the cardiac alpha-myosin heavy chain gene is controlled by a downstream repressor element containing a palindrome of two ets-binding sites.Molecular and cellular biology · 1998Article
- Transcription enhancer factor 1 interacts with a basic helix-loop-helix zipper protein, Max, for positive regulation of cardiac alpha-myosin heavy-chain gene expression.Molecular and cellular biology · 1997Article
- Differential expression of the myocyte enhancer factor 2 family of transcription factors in development: the cardiac factor BBF-1 is an early marker for cardiogenesis.Molecular and cellular biology · 1994Article
- A novel myogenic regulatory circuit controls slow/cardiac troponin C gene transcription in skeletal muscle.Molecular and cellular biology · 1994Article
- Negative regulation of transcription in eukaryotes.The Biochemical journal · 1993Review
- Identification of a functional silencer element involved in neuron-specific expression of the synapsin I gene.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- A new serum-responsive, cardiac tissue-specific transcription factor that recognizes the MEF-2 site in the myosin light chain-2 promoter.Molecular and cellular biology · 1993Article
- Identification of a negative element in the human vimentin promoter: modulation by the human T-cell leukemia virus type I Tax protein.Molecular and cellular biology · 1993Article
- Characterization of cis-acting elements regulating transcription from the promoter of a constitutively active rice actin gene.Molecular and cellular biology · 1992Article
- A single transcription factor binds to two divergent sequence elements with a common function in cardiac myosin light chain-2 promoter.Molecular and cellular biology · 1992Article
- Mechanism of tissue-specific transcription: interplay between positive and negative regulatory factors.Gene expression · 1992Article
- A position-dependent silencer plays a major role in repressing alpha-fetoprotein expression in human hepatoma.Molecular and cellular biology · 1991Article
- Myogenin induces the myocyte-specific enhancer binding factor MEF-2 independently of other muscle-specific gene products.Molecular and cellular biology · 1991Article
- Different mechanisms regulate muscle-specific AChR gamma- and epsilon-subunit gene expression.The EMBO journal · 1991Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Physiological expression of the cardiac muscle myosin light-chain 2 (MLC-2) gene in chickens is restricted to cardiac muscle tissue only, at least during the late embryonic to adult stages of development. The mechanism by which cardiac MLC-2 gene expression is repressed in differentiated noncardiac muscle tissues is unknown. Using sequential 5'-deletion mutants of the cardiac MLC-2 promoter introduced into primary skeletal muscle cells in culture, we have demonstrated that a 89-bp region, designated the cardiac-specific sequence (CSS), is essential for repression of cardiac MLC-2 expression in skeletal muscle. Removal of the CSS sequence alone allows transcription in skeletal muscle cells without affecting the transcriptional activity of the promoter in cardiac muscle cells. DNase I footprinting and gel shift assays indicate that protein binding to sequences in the CSS domain occurs readily in nuclear extracts obtained from skeletal muscle but not in extracts isolated under identical conditions from cardiac muscle. Thus, it appears that a negative regulatory mechanism accounts for the lack of expression of the cardiac MLC-2 gene in skeletal muscle and that the CSS element and its binding proteins are important functional components of the regulatory apparatus which ensures the developmental program for cardiac tissue-specific gene expression.
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