ArticleAnalytical and bioanalytical chemistry2010
A theoretical introduction to "combinatory SYBRGreen qPCR screening", a matrix-based approach for the detection of materials derived from genetically modified plants.
Article in Analytical and bioanalytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed.
- Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system.Applied microbiology and biotechnology · 2018Article
- Article
- Perspectives on genetically modified crops and food detection.Journal of food and drug analysis · 2016Review
- A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium.Applied microbiology and biotechnology · 2015Article
- Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.Applied microbiology and biotechnology · 2015Article
- New multiplex PCR methods for rapid screening of genetically modified organisms in foods.Frontiers in microbiology · 2015Article
- Current and new approaches in GMO detection: challenges and solutions.BioMed research international · 2015Review
- Development of a general method for detection and quantification of the P35S promoter based on assessment of existing methods.Scientific reports · 2014Article
- GMOseek: a user friendly tool for optimized GMO testing.BMC bioinformatics · 2014Article
- SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels.Applied microbiology and biotechnology · 2013Article
- The GMOseek matrix: a decision support tool for optimizing the detection of genetically modified plants.BMC bioinformatics · 2013Article
- How to deal with the upcoming challenges in GMO detection in food and feed.Journal of biomedicine & biotechnology · 2012Review
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7 authors.
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Abstract
The detection of genetically modified (GM) materials in food and feed products is a complex multi-step analytical process invoking screening, identification, and often quantification of the genetically modified organisms (GMO) present in a sample. "Combinatory qPCR SYBRGreen screening" (CoSYPS) is a matrix-based approach for determining the presence of GM plant materials in products. The CoSYPS decision-support system (DSS) interprets the analytical results of SYBRGREEN qPCR analysis based on four values: the C(t)- and T(m) values and the LOD and LOQ for each method. A theoretical explanation of the different concepts applied in CoSYPS analysis is given (GMO Universe, "Prime number tracing", matrix/combinatory approach) and documented using the RoundUp Ready soy GTS40-3-2 as an example. By applying a limited set of SYBRGREEN qPCR methods and through application of a newly developed "prime number"-based algorithm, the nature of subsets of corresponding GMO in a sample can be determined. Together, these analyses provide guidance for semi-quantitative estimation of GMO presence in a food and feed product.
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