ArticleThe Journal of biological chemistry2010
The TGL2 gene of Saccharomyces cerevisiae encodes an active acylglycerol lipase located in the mitochondria.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 42 citations in OpenAlex.
- Engineering yeast for tailored fatty acid profiles.Applied microbiology and biotechnology · 2025Review
- Exogenous Regulators Enhance the Yield and Stress Resistance of Chlamydospores of the Biocontrol AgentJournal of fungi (Basel, Switzerland) · 2022Article
- Mucor circinelloides: a model organism for oleaginous fungi and its potential applications in bioactive lipid production.Microbial cell factories · 2022Review
- Oxidative Stress Responses and Nutrient Starvation in MCHM TreatedG3 (Bethesda, Md.) · 2020Article
- The mitochondrial intermembrane space-facing proteins Mcp2 and Tgl2 are involved in yeast lipid metabolism.Molecular biology of the cell · 2019Article
- Article
- Label-free measurement of the yeast short chain TAG lipase activity by ESI-MS after one-step esterification.Journal of lipid research · 2017Article
- A molecular genetic toolbox forBiotechnology for biofuels · 2017Article
- Lipase genes in Mucor circinelloides: identification, sub-cellular location, phylogenetic analysis and expression profiling during growth and lipid accumulation.Journal of industrial microbiology & biotechnology · 2016Article
- Modifications of the C terminus affect functionality and stability of yeast triacylglycerol lipase Tgl3p.The Journal of biological chemistry · 2014Article
- The path to triacylglyceride obesity in the sta6 strain of Chlamydomonas reinhardtii.Eukaryotic cell · 2014Article
- Revising the Representation of Fatty Acid, Glycerolipid, and Glycerophospholipid Metabolism in the Consensus Model of Yeast Metabolism.Industrial biotechnology (New Rochelle, N.Y.) · 2013Article
- Review
- Neutral lipid metabolism influences phospholipid synthesis and deacylation in Saccharomyces cerevisiae.PloS one · 2012Article
- The inhibitory effect of cerulenin to yeasts is fungicidal.Communicative & integrative biology · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Saccharomyces cerevisiae Tgl2 protein shows sequence homology to Pseudomonas triacylglycerol (TAG) lipases, but its role in the yeast lipid metabolism is not known. Using hemagglutinin-tagged Tgl2p purified from yeast, we report that this protein carries a significant lipolytic activity toward long-chain TAG. Importantly, mutant hemagglutinin-Tgl2p(S144A), which contains alanine 144 in place of serine 144 in the lipase consensus sequence (G/A)XSXG exhibits no such activity. Although cellular TAG hydrolysis is reduced in the tgl2 deletion mutant, overproduction of Tgl2p in this mutant leads to an increase in TAG degradation in the presence of fatty acid synthesis inhibitor cerulenin, but that of Tgl2p(S144A) does not. This result demonstrates the lipolytic function of Tgl2p in yeast. Although other yeast TAG lipases are localized to lipid particles, Tgl2p is enriched in the mitochondria. The mitochondrial fraction purified from the TGL2-overexpressing yeast shows a strong lipolytic activity, which was absent in the tgl2 deletion mutant. Therefore, we conclude that Tgl2p is a functional lipase of the yeast mitochondria. By analyzing phenotypic effects of TGL2-deficient yeast, we also find that lipolysis-competent Tgl2p is required for the viability of cells treated with antimitotic drug. The addition of oleic acid, the product of Tgl2p-catalyzed lipolysis, fully complements the antimitotic drug sensitivity of the tgl2 null mutation. Thus, we propose that the mitochondrial Tgl2p-dependent lipolysis is crucial for the survival of cells under antimitotic drug treatment.
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