ArticleThe American journal of pathology2010
Differential effects of myocilin and optineurin, two glaucoma genes, on neurite outgrowth.
Article in The American journal of pathology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Common genetic determinants of intraocular pressure and primary open-angle glaucoma.PLoS genetics · 2012Pooled it
- Glaucoma Pathogenesis and Neurotrophins: Focus on the Molecular and Genetic Basis for Therapeutic Prospects.Current neuropharmacology · 2018Review
- FOXC1 modulates MYOC secretion through regulation of the exocytic proteins RAB3GAP1, RAB3GAP2 and SNAP25.PloS one · 2017Article
- Mitochondrial pathogenic mechanism and degradation in optineurin E50K mutation-mediated retinal ganglion cell degeneration.Scientific reports · 2016Article
- Optineurin: The autophagy connection.Experimental eye research · 2016Review
- Genes, pathways, and animal models in primary open-angle glaucoma.Eye (London, England) · 2015Review
- Induction of autophagy in rats upon overexpression of wild-type and mutant optineurin gene.BMC cell biology · 2015Article
- Focus on molecular events in the anterior chamber leading to glaucoma.Cellular and molecular life sciences : CMLS · 2014Review
- The nerve growth factor signaling and its potential as therapeutic target for glaucoma.BioMed research international · 2014Review
- Loss of optineurin in vivo results in elevated cell death and alters axonal trafficking dynamics.PloS one · 2014Article
- Effects of mutations and deletions in the human optineurin gene.SpringerPlus · 2014Article
- Current concepts on primary open-angle glaucoma genetics: a contribution to disease pathophysiology and future treatment.Eye (London, England) · 2012Review
- Cellular and molecular biology of optineurin.International review of cell and molecular biology · 2012Review
- Establishment of inducible wild type and mutant myocilin-GFP-expressing RGC5 cell lines.PloS one · 2012Article
- Myocilin, a glaucoma-associated protein, promotes cell migration through activation of integrin-focal adhesion kinase-serine/threonine kinase signaling pathway.Journal of cellular physiology · 2011Article
- Myocilin and Optineurin: Differential Characteristics and Functional Consequences.Taiwan journal of ophthalmology · 2011Article
- Processing of optineurin in neuronal cells.The Journal of biological chemistry · 2011Article
- Effects of optineurin siRNA on apoptotic genes and apoptosis in RGC-5 cells.Molecular vision · 2011Article
- Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Myocilin and optineurin are two genes linked to glaucoma, a major blinding disease characterized by progressive loss of retinal ganglion cells (RGCs) and their axons. To investigate the effects of force-expressed wild-type and mutant myocilin and optineurin on neurite outgrowth in neuronal cells, we transiently transfected cells with pEGFP-N1 (mock control) as well as myocilin and optineurin plasmids including pMYOC(WT)-EGFP, pMYOC(P370L)-EGFP, pMYOC(1-367)-EGFP, pOPTN(WT)-EGFP, and pOPTN(E50K)-EGFP. PC12 cells transfected with pEGFP-N1 produced, as anticipated, long and extensive neuritis on nerve growth factor induction. The neurite length in those cells transfected with myocilin constructs was shortened and the number of neurites was also reduced. A similar inhibitory effect on neurite outgrowth was also elicited by myocilin transfection in RGC5 cells. In contrast, neither transfection of the optineurin constructs pOPTN(WT)-EGFP and pOPTN(E50K)-EGFP nor the myocilin and optineurin small-interfering RNA treatments induced significant alterations in neurite outgrowth. Transfection with the wild-type optineurin construct, but not with that of the wild-type myocilin, increased the apoptotic activity in cells. These results demonstrated that the two glaucoma genes, myocilin and optineurin, exhibited differential effects on neurite outgrowth. They may contribute to the development of neurodegenerative glaucoma via distinct mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.