ArticleThe American journal of sports medicine2010
Effects of dexamethasone on the functional properties of cartilage explants during long-term culture.
Article in The American journal of sports medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 48 citations in OpenAlex.
- Click Chemistry-Based Quantification of Extracellular Matrix Turnover for Drug Screening and Regenerative Medicine.Annals of biomedical engineering · 2026Article
- Evaluation of Dexamethasone-Eluting Cell-Seeded Constructs in a Preclinical Canine Model of Cartilage Repair.Tissue engineering. Part A · 2025Article
- Triamcinolone acetonide has minimal effect on short- and long-term metabolic activities of cartilage.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2024Article
- Screening of poly-beta amino ester coated emulsion of ketorolac for cartilage delivery.Journal of materials chemistry. B · 2024Article
- Modernizing Storage Conditions for Fresh Osteochondral Allografts by Optimizing Viability at Physiologic Temperatures and Conditions.Cartilage · 2021Article
- Ex Vivo Systems to Study Chondrogenic Differentiation and Cartilage Integration.Journal of functional morphology and kinesiology · 2021Review
- Protocol for Harvest, Transport and Storage of Human Osteochondral Tissue.Revista brasileira de ortopedia · 2020Article
- Sustained low-dose dexamethasone delivery via a PLGA microsphere-embedded agarose implant for enhanced osteochondral repair.Acta biomaterialia · 2020Article
- Dexamethasone: chondroprotective corticosteroid or catabolic killer?European cells & materials · 2019Review
- Identification of Chondrocyte Genes and Signaling Pathways in Response to Acute Joint Inflammation.Scientific reports · 2019Article
- Calcium signaling of in situ chondrocytes in articular cartilage under compressive loading: Roles of calcium sources and cell membrane ion channels.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- Concise Review: Mesenchymal Stem Cells for Functional Cartilage Tissue Engineering: Taking Cues from Chondrocyte-Based Constructs.Stem cells translational medicine · 2017Review
- Cartilage storage at 4 °C with regular culture medium replacement benefits chondrocyte viability of osteochondral grafts in vitro.Cell and tissue banking · 2016Article
- Inhibition of CDK9 prevents mechanical injury-induced inflammation, apoptosis and matrix degradation in cartilage explants.European cells & materials · 2016Article
- Long-term storage and preservation of tissue engineered articular cartilage.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2016Article
- Impact of storage conditions on electromechanical, histological and histochemical properties of osteochondral allografts.BMC musculoskeletal disorders · 2015Article
- Fabrication of tissue engineered osteochondral grafts for restoring the articular surface of diarthrodial joints.Methods (San Diego, Calif.) · 2015Article
- The effect of chemically defined medium on spontaneous calcium signaling of in situ chondrocytes during long-term culture.Journal of biomechanics · 2015Article
- Effects of insulin-like growth factor-1 and dexamethasone on cytokine-challenged cartilage: relevance to post-traumatic osteoarthritis.Osteoarthritis and cartilage · 2015Article
- Trimethylamine N-oxide as a media supplement for cartilage tissue engineering.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundIntact articular cartilage tissue is used clinically in the form of osteochondral allografts and experimentally as explants in modeling the physiologic behavior of chondrocytes in their native extracellular matrix. Long-term maintenance of allograft tissue is challenging. HYPOTHESIS: By carefully modulating the preservation environment, it may be possible to preserve osteochondral allograft tissue over the long term while maintaining its original mechanical and biochemical properties. STUDY
designControlled laboratory study.
methodsIn this study, juvenile bovine, mature bovine, and canine cartilage explants were cultured in chemically defined media with or without supplementation of dexamethasone for up to 4 weeks.
resultsThe mechanical properties and biochemical content of juvenile bovine explants cultured in the presence of dexamethasone were significantly enhanced after 2 weeks in culture and remained stable with sustained cell viability thereafter. In contrast, the mechanical properties and biochemical content of juvenile bovine explants cultured in the absence of the dexamethasone significantly decreased after 2 weeks of culture. The mechanical and biochemical content of mature bovine and canine explants were not significantly affected by the presence of dexamethasone and maintained initial (day 0) mechanical and biochemical properties throughout the entire culture period with or without supplementation of dexamethasone.
conclusionThese results suggest that juvenile and mature cartilage explants respond differently to dexamethasone. The functional properties of juvenile cartilage explants can be maintained in vitro through the addition of dexamethasone to culture media. Functional properties of mature cartilage can be preserved for at least 4 weeks in culture regardless of the presence of dexamethasone. CLINICAL RELEVANCE: Biochemical and biomechanical properties of osteochondral allograft tissue may be enhanced by the addition of dexamethasone to culture media. These findings may translate to longer shelf life of preserved osteochondral allograft transplantation tissue and increased clinical availability of grafts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.