ArticleInvestigative ophthalmology & visual science2010
Tear lipocalin captures exogenous lipid from abnormal corneal surfaces.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Evidence for Phospholipids on the Surface of Human Tears.Investigative ophthalmology & visual science · 2020Article
- Ligand binding complexes in lipocalins: Underestimation of the stoichiometry parameter (n).Biochimica et biophysica acta. Proteins and proteomics · 2018Article
- Interaction of ceramides and tear lipocalin.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2018Article
- Tear lipocalin, lysozyme and lactoferrin concentrations in postmenopausal women.Journal of medicine and life · 2015Article
- Lipidomic analysis of human tear fluid reveals structure-specific lipid alterations in dry eye syndrome.Journal of lipid research · 2014Article
- Practical issues concerning tear protein assays in dry eye.Eye and vision (London, England) · 2014Review
- Article
- Tear film lipids.Experimental eye research · 2013Review
- A novel fluorescent lipid probe for dry eye: retrieval by tear lipocalin in humans.Investigative ophthalmology & visual science · 2013Article
- Article
- Tryptophan rotamer distribution revealed for the α-helix in tear lipocalin by site-directed tryptophan fluorescence.The journal of physical chemistry. B · 2012Article
- Cation-π interactions in lipocalins: structural and functional implications.Biochemistry · 2012Article
- Mass spectrometric identification of phospholipids in human tears and tear lipocalin.Investigative ophthalmology & visual science · 2012Article
- Tear lipocalin: structure and function.The ocular surface · 2011Review
- Fluorescein punctate staining traced to superficial corneal epithelial cells by impression cytology and confocal microscopy.Investigative ophthalmology & visual science · 2011Article
- Focus on molecules: tear lipocalin.Experimental eye research · 2011Review
- Interaction of phospholipid transfer protein with human tear fluid mucins.Journal of lipid research · 2010Article
- Excited protein states of human tear lipocalin for low- and high-affinity ligand binding revealed by functional AB loop motion.Biophysical chemistry · 2010Article
- Correlations between eyelid tumors and tear lipocalin, lysozyme and lactoferrin concentrations in postmenopausal women.Journal of medicine and lifeArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Purpose. The cornea is protected by apical hydrophilic transmembrane mucins and tears. In pathologic states the mucin barrier is disrupted, creating potential for meibomian lipids to adhere more strongly. Undisplaced lipids create an unwettable surface. The hypothesis that pathologic ocular surfaces alter lipid binding and the ability of tear proteins to remove lipids was tested. Methods. Corneas with pathologic surfaces were studied for lipid adhesion and removal by tears. Capture of fluorescence-labeled phospholipids by human tears was assessed by steady state fluorometry. Tear proteins were separated by gel filtration chromatography and analyzed for bound lipids. Results. Contact angle measurements revealed strong lipid adherence to corneas submerged in buffer. Lower contact angles are observed for lipids on completely de-epithelialized corneas compared with intact corneas (P = 0.04). Lipid removal from these surfaces is greater with whole tears than with tears depleted of tear lipocalin (P < 0.0005). Significantly fewer lipids are captured by tears from Bowman's layer than from epithelial-bearing surfaces (P < 0.025). The only tear component to bind the fluorescence-tagged lipid is tear lipocalin. The histology of a rare case of dry eye disease demonstrates the dominant features of contemporaneous bullous keratopathy. Lipid sequestration from this cornea by tear lipocalin was robust. Conclusions. Lipid is captured by tear lipocalin from corneas with bullous keratopathy and dry eye. Lipid removal is slightly abrogated by greater lipid adhesion to Bowman's layer. Reduced secretion of tear lipocalin documented in dry eye disease could hamper lipid removal and exacerbate ocular surface pathology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.