ArticleThe Journal of biological chemistry2010
Comparative study of the gating motif and C-type inactivation in prokaryotic voltage-gated sodium channels.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Nemertide Alpha-1 as a Biopesticide: Aphid Deterrence, Antimicrobial Activity, and Safety Aspects.Marine drugs · 2025Article
- Psychological stress-induced ALKBH5 deficiency promotes tumour innervation and pancreatic cancer via extracellular vesicle transfer of RNA.Nature cell biology · 2025Article
- The chemistry of electrical signaling in sodium channels from bacteria and beyond.Cell chemical biology · 2024Review
- Honeybee CaV4 has distinct permeation, inactivation, and pharmacology from homologous NaV channels.The Journal of general physiology · 2024Article
- The structural basis of divalent cation block in a tetrameric prokaryotic sodium channel.Nature communications · 2023Article
- When the Gates Swing Open Only: Arrhythmia Mutations That Target the Fast Inactivation Gate of NaCells · 2022Review
- N-type fast inactivation of a eukaryotic voltage-gated sodium channel.Nature communications · 2022Article
- Engineered bacterial voltage-gated sodium channel platform for cardiac gene therapy.Nature communications · 2022Article
- An open state of a voltage-gated sodium channel involving a π-helix and conserved pore-facing asparagine.Biophysical journal · 2022Article
- Druggability of Voltage-Gated Sodium Channels-Exploring Old and New Drug Receptor Sites.Frontiers in pharmacology · 2022Review
- Review
- Sympathetic and parasympathetic innervation in cancer: therapeutic implications.Clinical autonomic research : official journal of the Clinical Autonomic Research Society · 2021Review
- Brevetoxin and Conotoxin Interactions with Single-Domain Voltage-Gated Sodium Channels from a Diatom and Coccolithophore.Marine drugs · 2021Article
- In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapy.Methods in enzymology · 2021Article
- The insights into calcium ion selectivity provided by ancestral prokaryotic ion channels.Biophysics and physicobiology · 2021Article
- A Novel Single-Domain NaPlant physiology · 2020Article
- A native prokaryotic voltage-dependent calcium channel with a novel selectivity filter sequence.eLife · 2020Article
- Extremely Potent Block of Bacterial Voltage-Gated Sodium Channels by µ-Conotoxin PIIIA.Marine drugs · 2019Article
- Genetic manipulation of autonomic nerve fiber innervation and activity and its effect on breast cancer progression.Nature neuroscience · 2019Article
- Alternative Mechanisms for Fast NaCurrent biology : CB · 2019Article
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6 authors.
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Abstract
Prokaryotic voltage-gated sodium channels (Na(V)s) are homotetramers and are thought to inactivate through a single mechanism, named C-type inactivation. Here we report the voltage dependence and inactivation rate of the NaChBac channel from Bacillus halodurans, the first identified prokaryotic Na(V), as well as of three new homologues cloned from Bacillus licheniformis (Na(V)BacL), Shewanella putrefaciens (Na(V)SheP), and Roseobacter denitrificans (Na(V)RosD). We found that, although activated by a lower membrane potential, Na(V)BacL inactivates as slowly as NaChBac. Na(V)SheP and Na(V)RosD inactivate faster than NaChBac. Mutational analysis of helix S6 showed that residues corresponding to the "glycine hinge" and "PXP motif" in voltage-gated potassium channels are not obligatory for channel gating in these prokaryotic Na(V)s, but mutations in the regions changed the inactivation rates. Mutation of the region corresponding to the glycine hinge in Na(V)BacL (A214G), Na(V)SheP (A216G), and NaChBac (G219A) accelerated inactivation in these channels, whereas mutation of glycine to alanine in the lower part of helix S6 in NaChBac (G229A), Na(V)BacL (G224A), and Na(V)RosD (G217A) reduced the inactivation rate. These results imply that activation gating in prokaryotic Na(V)s does not require gating motifs and that the residues of helix S6 affect C-type inactivation rates in these channels.
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