ArticleMethods in molecular biology (Clifton, N.J.)2010
Manipulation of neutrophil-like HL-60 cells for the study of directed cell migration.
Article in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The trial behind it
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Who cites it
52 citing papers in PubMed, 85 citations in OpenAlex.
- Tissue flow acts as a guidance cue for immune cell polarization and directional migration.Nature cell biology · 2026Article
- PIP5K-Ras bistability triggers plasma membrane symmetry breaking to define cellular polarity and regulate migration.Nature communications · 2026Article
- Machine Learning-Based Detection ofPathogens (Basel, Switzerland) · 2026Article
- An immune-associated mitochondrial DNA variant with sex differences reveals a putative novel microprotein called MASL.Immunity & ageing : I & A · 2026Article
- Human cathelicidin peptide LL-37 compacts nucleic acids and alters neutrophil extracellular trap structure.Scientific reports · 2026Article
- Article
- Investigation of Regulation and Binding Patterns of the Human Cathelicidin Peptide LL-37 in Complexation with Nucleic Acids, and its Impact on Neutrophil Extracellular Traps.bioRxiv : the preprint server for biology · 2026Article
- PIP5K-Ras bistability triggers plasma membrane symmetry breaking to define cellular polarity and regulate migration.bioRxiv : the preprint server for biology · 2025Article
- Exosomal miR-216a-5p targeting TLR-4 alleviates liver ischemia-reperfusion injury by regulating M2 macrophage polarization and neutrophil inflammation.Annals of medicine · 2025Article
- A bipartite bacterial virulence factor targets the complement system and neutrophil activation.The EMBO journal · 2025Article
- Ex Vivo Delivery of mRNA to Immune Cells via a Nonendosomal Route Obviates the Need for Nucleoside Modification.ACS bio & med chem Au · 2024Article
- pH-Dependent migratory behaviors of neutrophil-like cells in a microfluidic device with controllability of dissolved gas concentrations.Scientific reports · 2024Article
- PGRMC2 and HLA-G regulate immune homeostasis in a microphysiological model of human maternal-fetal membrane interface.Communications biology · 2024Article
- Metabolic features of neutrophilic differentiation of HL-60 cells in hyperglycemic environments.BMJ open diabetes research & care · 2024Article
- Ras suppression potentiates rear actomyosin contractility-driven cell polarization and migration.Nature cell biology · 2024Article
- Article
- trans-Endothelial neutrophil migration activates bactericidal function via Piezo1 mechanosensing.Immunity · 2024Article
- Culture and Maintenance of Immune Cells to Model Innate Immune Status at the Feto-maternal Interface.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A dynamic partitioning mechanism polarizes membrane protein distribution.Nature communications · 2023Article
- Dual Affinity Nanoparticles for the Transport of Therapeutics from Carrier Cells to Target Cells under Physiological Flow Conditions.ACS omega · 2023Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Many cells undergo directed cell migration in response to external cues in a process known as chemotaxis. This ability is essential for many single-celled organisms to hunt and mate, the development of multicellular organisms, and the functioning of the immune system. Because of their relative ease of manipulation and their robust chemotactic abilities, the neutrophil-like cell line (HL-60) has been a powerful system to analyze directed cell migration. In this chapter, we describe the maintenance and transient transfection of HL-60 cells and explain how to analyze their behavior with two standard chemotactic assays (micropipette and EZ-TAXIS). Finally, we demonstrate how to fix and stain the actin cytoskeleton of polarized cells for fluorescent microscopy imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.