ArticleMarine biotechnology (New York, N.Y.)2010
Construction of two BAC libraries from half-smooth tongue sole Cynoglossus semilaevis and identification of clones containing candidate sex-determination genes.
Article in Marine biotechnology (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed.
- Unveiling Gene Expression Dynamics during Early Embryogenesis inLife (Basel, Switzerland) · 2024Article
- Seminal Plasma Exosomes: Promising Biomarkers for Identification of Male and Pseudo-Males in Cynoglossus semilaevis.Marine biotechnology (New York, N.Y.) · 2019Article
- Construction of a Genomic Bacterial Artificial Chromosome (BAC) Library for the Prawn Macrobrachium rosenbergii and Initial Analysis of ZW Chromosome-Derived BAC Inserts.Marine biotechnology (New York, N.Y.) · 2019Article
- The transcriptomic signature of different sexes in two protogynous hermaphrodites: Insights into the molecular network underlying sex phenotype in fish.Scientific reports · 2018Article
- The Gene Toolkit Implicated in Functional Sex in Sparidae Hermaphrodites: Inferences From Comparative Transcriptomics.Frontiers in genetics · 2018Article
- Characterization of a low-density lipoprotein receptor, Lrp13, in Chinese tongue sole (Cynoglossus semilaevis) and medaka (Oryzias latipes).Fish physiology and biochemistry · 2017Article
- Identification of exosomes and its signature miRNAs of male and female Cynoglossus semilaevis.Scientific reports · 2017Article
- Molecular characterization of three gonadotropin subunits and their expression patterns during ovarian maturation in Cynoglossus semilaevis.International journal of molecular sciences · 2015Article
- Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones.Chromosoma · 2014Article
- A first generation BAC-based physical map of the half-smooth tongue sole (Cynoglossus semilaevis) genome.BMC genomics · 2014Article
- Whole-genome sequence of a flatfish provides insights into ZW sex chromosome evolution and adaptation to a benthic lifestyle.Nature genetics · 2014Article
- Cloning and characterization of tesk1, a novel spermatogenesis-related gene, in the tongue sole (Cynoglossus semilaevis).PloS one · 2014Article
- Male-biased genes in catfish as revealed by RNA-Seq analysis of the testis transcriptome.PloS one · 2013Article
- Genomic approaches to study genetic and environmental influences on fish sex determination and differentiation.Marine biotechnology (New York, N.Y.) · 2012Review
- Construction of BIBAC and BAC libraries from a variety of organisms for advanced genomics research.Nature protocols · 2012 · on this mapArticle
- Preparation of megabase-sized DNA from a variety of organisms using the nuclei method for advanced genomics research.Nature protocols · 2012Article
- Article
- Construction and characterization of the BAC library for common carp Cyprinus carpio L. and establishment of microsynteny with zebrafish Danio rerio.Marine biotechnology (New York, N.Y.) · 2011Article
- The birds and the bees and the flowers and the trees: lessons from genetic mapping of sex determination in plants and animals.Genetics · 2010Review
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7 authors.
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Abstract
Half-smooth tongue sole (Cynoglossus semilaevis) is an increasingly important aquaculture species in China. It is also a tractable model to study sex chromosome evolution and to further elucidate the mechanism of sex determination in teleosts. Two bacterial artificial chromosome (BAC) libraries for C. semilaevis, with large, high-quality inserts and deep coverage, were constructed in the BamHI and HindIII sites of the vector pECBAC1. The two libraries contain a total of 55,296 BAC clones arrayed in 144 384-well microtiter plates and correspond to 13.36 haploid genome equivalents. The combined libraries have a greater than 99% probability of containing any single-copy sequence. Screening high-density arrays of the libraries with probes for female-specific markers and sex-related genes generated between 4-46 primary positive clones per probe. Thus, the two BAC libraries of C. semilaevis provided a readily useable platform for genomics research, illustrated by the isolation of sex determination gene(s).
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