ArticlePlant cell reports2010
Expression of a glycosylated GFP as a bivalent reporter in exocytosis.
Article in Plant cell reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 17 citations in OpenAlex.
- ABC1K2 is involved in stress response and secondary metabolism during seed development in Arabidopsis thaliana.Plant cell reports · 2025Article
- TCMP-2 affects tomato flowering and interacts with BBX16, a homolog of the arabidopsis B-box MiP1b.Plant direct · 2020Article
- Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking.The Journal of biological chemistry · 2020Article
- CesA6 and PGIP2 Endocytosis Involves Different Subpopulations of TGN-Related Endosomes.Frontiers in plant science · 2020Article
- N-Linked Glycosylation Modulates Golgi-Independent Vacuolar Sorting Mediated by the Plant Specific Insert.Plants (Basel, Switzerland) · 2019Article
- Glutathione S-transferase related detoxification processes are correlated with receptor-mediated vacuolar sorting mechanisms.Plant cell reports · 2017Article
- '2A-Like' Signal Sequences Mediating Translational Recoding: A Novel Form of Dual Protein Targeting.Traffic (Copenhagen, Denmark) · 2016Article
- Delivering of proteins to the plant vacuole--an update.International journal of molecular sciences · 2014Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The complex-type N-linked glycans of plants differ markedly in structure from those of animals. Like those of insects and mollusks they lack terminal sialic acid(s) and may contain an alpha-(1,3)-fucose (Fuc) linked to the proximal GlcNAc residue and/or a beta-(1,2)-xylose (Xyl) residue attached to the proximal mannose (Man) of the glycan core. N-glycosylated GFPs were used in previous studies showing their effective use to report on membrane traffic between the ER and the Golgi apparatus in plant cells. In all these cases glycosylated tags were added at the GFP termini. Because of the position of the tag and depending on the sorting and accumulation site of these modified GFP, there is always a risk of processing and degradation, and this protein design cannot be considered ideal. Here, we describe the development of three different GFPs in which the glycosylation site is internally localized at positions 80, 133, or 172 in the internal sequence. The best glycosylation site was at position 133. This glycosylated GFPgl133 appears to be protected from undesired processing of the glycosylation site and represents a bivalent reporter for biochemical and microscopic studies. After experimental validation, we can conclude that amino acid 133 is an effective glycosylation site and that the GFPgl133 is a powerful tool for in vivo investigations in plant cell biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.