ArticleArchives of dermatological research2010
A novel human skin chamber model to study wound infection ex vivo.
Article in Archives of dermatological research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Article
- 3D Approaches to Culturing Bovine Skin: Explant Culture versus Organotypic Skin Model.Cells, tissues, organs · 2024Article
- Article
- Article
- Development ofMolecular pharmaceutics · 2023Article
- Maggot Extract Interrupts Bacterial Biofilm Formation and Maturation in Combination with Antibiotics by Reducing the Expression of Virulence Genes.Life (Basel, Switzerland) · 2022Article
- Optimising soft tissue in-growthBiomaterials translational · 2022Article
- Mesenchymal stromal cell-secreted CCL2 promotes antibacterial defense mechanisms through increased antimicrobial peptide expression in keratinocytes.Stem cells translational medicine · 2021Article
- Simultaneous Delivery of Multiple Antimicrobial Agents by Biphasic Scaffolds for Effective Treatment of Wound Biofilms.Advanced healthcare materials · 2021Article
- Polyphosphazenes enable durable, hemocompatible, highly efficient antibacterial coatings.Biomaterials · 2021Article
- Review
- Article
- Evaluation of Drug Delivery and Efficacy of Ciprofloxacin-Loaded Povidone Foils and Nanofiber Mats in a Wound-Infection Model Based on Ex Vivo Human Skin.Pharmaceutics · 2019Article
- A tractable, simplified ex vivo human skin model of wound infection.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2019Article
- In vitro and ex vivo systems at the forefront of infection modeling and drug discovery.Biomaterials · 2019Review
- Bioengineered Platforms for Chronic Wound Infection Studies: How Can We Make Them More Human-Relevant?Frontiers in bioengineering and biotechnology · 2019Review
- A Novel 3D Skin Explant Model to Study Anaerobic Bacterial Infection.Frontiers in cellular and infection microbiology · 2017Article
- Innate immune response after adenoviral gene delivery into skin is mediated by AIM2, NALP3, DAI and mda5.SpringerPlus · 2013Article
- Corrigendum: Bioengineered Platforms for Chronic Wound Infection Studies: How Can We Make Them More Human-Relevant?Frontiers in bioengineering and biotechnologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wound infections with multi-drug resistant bacteria increase morbidity and mortality and have considerable socioeconomic impact. They can lead to impaired wound healing, resulting in rising treatment costs. The aim of this study was to investigate an ex vivo human wound infection model. Human full-thickness skin from the operating room (OR) was placed into the Bo-Drum and cultivated for 7 days in an air-liquid interphase. On day 8, the skin was inoculated with either (1) Pseudomonas aeruginosa, (2) Staphylococcus aureus (10(5) CFU, n = 3) or (3) carrier control. 1, 3 and 7 days after inoculation colony forming units in the tissue/media were determined and cytokine expression was quantified. A reliable and reproducible wound infection could be established for 7 days. At this time point, 1.8 x 10(8) CFU/g tissue of P. aeruginosa and 2 x 10(7) CFU/g tissue of S. aureus were detected. Immunohistochemical analysis demonstrated bacterial infection and epidermolysis in infected skin. RT-PCR analysis exhibited a significant induction of proinflammatory cytokines after infection. The BO-drum is a robust, easy-to-use, sterilizable and reusable ex vivo full-skin culture system. For investigation of wound infection, treatment and healing, the BO-drum presents a convenient model and may help to standardize wound research.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.