ArticlePlant cell reports2010
Pre- and post-agroinfection strategies for efficient leaf disk transformation and regeneration of transgenic strawberry plants.
Article in Plant cell reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Role of non-coding RNAs in quality improvement of horticultural crops: computational tools, databases, and algorithms for identification and analysis.Functional & integrative genomics · 2025Review
- An Effective Somatic-Cell Regeneration and Genetic Transformation Method Mediated byPlants (Basel, Switzerland) · 2024Article
- An EfficientPlants (Basel, Switzerland) · 2024Article
- Advances in genomics and genome editing for improving strawberry (Frontiers in genetics · 2024Review
- Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method.Plant methods · 2023Article
- EfficientInternational journal of molecular sciences · 2019Article
- Development of transgenic broccoli with3 Biotech · 2018Article
- Efficient regeneration and improved sonication-assisted Agrobacterium transformation (SAAT) method for Catharanthus roseus.3 Biotech · 2017Article
- An efficient Agrobacterium-mediated transformation of strawberry cv. Camarosa by a dual plasmid system.Molecules (Basel, Switzerland) · 2015Article
- An efficient Agrobacterium-mediated transformation system for poplar.International journal of molecular sciences · 2014Article
- Article
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1 author.
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Abstract
Following previously described Agrobacterium tumefaciens-mediated transformation procedures for Fragaria x ananassa Duch. 'Chandler', we undertook several experiments to establish the importance of some parameters affecting transformation. The most important factor that increased the percent recovery of transformants was the introduction of a pre-selection phase, in-between co-cultivation and selection, in which leaf disks were cultured on pre-selection regeneration medium containing validamycin A, timentin, and cefotaxime. The average percentage of leaf disks forming shoots on selection medium containing cefotaxime (250 mg l(-1)) + timentin (250 mg l(-1)) was 5.4% and about three shoots per regenerating leaf disk. Maximum transformation percentage, based on polymerase chain reaction, was 31.25%. Transgene integration and copy number were assessed by Southern hybridization confirming single copy as well as multiple copies of transgene integration in shoots as well as roots separately. This confirmed the non-chimeric nature of these transgenic plants. The system is very promising for the regeneration of genetically transformed cells and obtaining transgenic strawberry plants at high efficiency.
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