ArticleGene therapy2010
Electroporation-mediated delivery of a naked DNA plasmid expressing VEGF to the porcine heart enhances protein expression.
Article in Gene therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 57 citations in OpenAlex.
- Conversion of natural cytokine receptors into orthogonal synthetic biosensors.Nature chemical biology · 2025Article
- Targeting calcium-mediated inter-organellar crosstalk in cardiac diseases.Expert opinion on therapeutic targets · 2022Article
- Recent advances in gene therapy for atrial fibrillation.Journal of cardiovascular electrophysiology · 2021Review
- Drop-to-drop liquid-liquid extraction of DNA in an electrowetting-on-dielectric digital microfluidics.Biomicrofluidics · 2021Article
- Targeted Knockout of the Vegfa Gene in the Retina by Subretinal Injection of RNP Complexes Containing Cas9 Protein and Modified sgRNAs.Molecular therapy : the journal of the American Society of Gene Therapy · 2021Article
- Therapeutic strategies for enhancing angiogenesis in wound healing.Advanced drug delivery reviews · 2019Review
- Cardiovascular Precision Medicine in the Genomics Era.JACC. Basic to translational science · 2018Review
- Getting Drugs Across Biological Barriers.Advanced materials (Deerfield Beach, Fla.) · 2017Review
- Electroporation-mediated gene delivery.Advances in genetics · 2015Article
- The Use of Gene Therapy for Ablation of Atrial Fibrillation.Arrhythmia & electrophysiology review · 2014Article
- Article
- Gene therapy delivery systems for enhancing viral and nonviral vectors for cardiac diseases: current concepts and future applications.Human gene therapy · 2013Review
- Cell-specific targeting strategies for electroporation-mediated gene delivery in cells and animals.The Journal of membrane biology · 2013Review
- Transgene delivery to endothelial cultures derived from porcine carotid artery ex vivo.Translational stroke research · 2013Article
- Gene therapy to treat cardiovascular disease.Journal of the American Heart Association · 2013Review
- Myocardial gene transfer: routes and devices for regulation of transgene expression by modulation of cellular permeability.Human gene therapy · 2013Review
- Electroporation-mediated gene transfer directly to the swine heart.Gene therapy · 2013Article
- Gene therapy approaches to regenerating bone.Advanced drug delivery reviews · 2012Review
- Muscle gene electrotransfer is increased by the antioxidant tempol in mice.Gene therapy · 2012Article
- In vivo site-specific transfection of naked plasmid DNA and siRNAs in mice by using a tissue suction device.PloS one · 2012Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
Abstract
Gene therapy is an attractive method for the treatment of cardiovascular disease. However, using current strategies, induction of gene expression at therapeutic levels is often inefficient. In this study, we show a novel electroporation (EP) method to enhance the delivery of a plasmid expressing an angiogenic growth factor (vascular endothelial growth factor, VEGF), which is a molecule previously documented to stimulate revascularization in coronary artery disease. DNA expression plasmids were delivered in vivo to the porcine heart with or without coadministered EP to determine the potential effect of electrically mediated delivery. The results showed that plasmid delivery through EP significantly increased cardiac expression of VEGF compared with injection of plasmid alone. This is the first report showing successful intracardiac delivery, through in vivo EP, of a protein expressing plasmid in a large animal.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.