ArticleIntervirology2010
Comprehensive detection and identification of seven animal coronaviruses and human respiratory coronavirus 229E with a microarray hybridization assay.
Article in Intervirology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 56 citations in OpenAlex.
- The application status of sequencing technology in global respiratory infectious disease diagnosis.Infection · 2024Review
- Advances in Laboratory Diagnosis of Coronavirus Infections in Cattle.Pathogens (Basel, Switzerland) · 2024Review
- Nanopore sequencing technology and its applications.MedComm · 2023Review
- Emerging COVID-19 variants and their impact on SARS-CoV-2 diagnosis, therapeutics and vaccines.Annals of medicine · 2022Review
- Advancements in Detection Approaches of Severe Acute Respiratory Syndrome Coronavirus 2.The Malaysian journal of medical sciences : MJMS · 2022Review
- Current trends in COVID-19 diagnosis and its new variants in physiological fluids: Surface antigens, antibodies, nucleic acids, and RNA sequencing.Trends in analytical chemistry : TRAC · 2022Review
- Era of Molecular Diagnostics Techniques before and after the COVID-19 Pandemic.Current issues in molecular biology · 2022Review
- Review
- Advances in Bovine Coronavirus Epidemiology.Viruses · 2022Review
- An Insight Into Detection Pathways/Biosensors of Highly Infectious Coronaviruses.Molecular biotechnology · 2022Review
- Comprehensive Review on Rapid Diagnosis of New Infection COVID-19.Applied biochemistry and biotechnology · 2022Review
- COVID-19 rhapsody: Rage towards advanced diagnostics and therapeutic strategy.Journal of pharmaceutical analysis · 2021Review
- Molecular diagnostic assays for COVID-19: an overview.Critical reviews in clinical laboratory sciences · 2021Review
- COVID-19 Diagnostic Strategies. Part I: Nucleic Acid-Based Technologies.Bioengineering (Basel, Switzerland) · 2021Review
- Recent trends in analytical and digital techniques for the detection of the SARS-Cov-2.Biophysical chemistry · 2021Review
- COVID-19: Virology, biology and novel laboratory diagnosis.The journal of gene medicine · 2021Review
- A Comprehensive Review of Detection Methods for SARS-CoV-2.Microorganisms · 2021Review
- Current Challenges for the Effective Management of the COVID-19 Pandemic.Advances in experimental medicine and biology · 2021Article
- A concise discussion on the potential spectral tools for the rapid COVID-19 detection.Results in chemistry · 2021Review
- Mapping the intellectual structure of the coronavirus field (2000-2020): a co-word analysis.Scientometrics · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Based on microarray hybridization, a diagnostic test for coronavirus infection was developed using eight coronavirus strains: canine coronavirus (CCoV), feline infectious peritonitis virus (FIPV), feline coronavirus (FCoV), bovine coronavirus (BCoV), porcine respiratory coronavirus (PRCoV), turkey enteritis coronavirus (TCoV), transmissible gastroenteritis virus (TGEV), and human respiratory coronavirus (HRCoV). Up to 104 cDNA clones of eight viruses were obtained by reverse transcription PCR with different pairs of primers designed for each virus and a pair of universal primers designed for the RNA polymerase gene of coronavirus. Total RNAs extracted from virus were reverse transcribed, followed by multi-PCR amplification and labeled with Cy3-dCTP. All labeled cDNAs and prepared gene chips were subjected to specific hybridization. The results showed that extensive cross-reaction existed between CCoV, FCoV, FIPV, TGEV and PRCoV, while there was no cross-reaction between BCoV, TCoV and HRCoV. The ultimate specific gene chip was developed with DNA fragments reamplified from the chosen recombinant plasmids without cross-reaction between different coronaviruses. The hybridization results showed that this gene chip could specifically identify and distinguish the eight coronaviruses and the sensitivity of the chip may be 1,000x more sensitive than PCR, indicating that it can be used for the diagnosis of eight coronavirus infections at the same time.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.