ArticleProceedings of the National Academy of Sciences of the United States of America2009
Unique charge distribution in surface loops confers high velocity on the fast motor protein Chara myosin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- The structure of the native cardiac crossbridge in the rigor state.Science advances · 2026Article
- The calmodulin-like proteins, CML13 and CML14 function as myosin light chains for the class XI myosins in Arabidopsis.Plant & cell physiology · 2026Article
- Elucidating chiral myosin-induced actin dynamics: From single-filament behavior to collective structures.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Carnosine enhances actomyosin-ATPase activity under acidic conditions: the role of carnosine in a rigor mortis.Journal of animal science · 2026Article
- Dynamics of full-length Arabidopsis myosin XI and its involvement in actin remodeling.Plant & cell physiology · 2025Article
- A FRET assay to monitor different structural states of human β-cardiac myosin including the interacting-heads motif.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Engineering filamentous myosins for optical control of contractility.bioRxiv : the preprint server for biology · 2025Article
- Functional characterization of calmodulin-like proteins, CML13 and CML14, as novel light chains of Arabidopsis class VIII myosins.Journal of experimental botany · 2024Article
- Unraveling the fastest myosin: Discovery history and structure-function relationships of algaeBiophysics and physicobiology · 2024Review
- Autoregulation and dual stepping mode of MYA2, an Arabidopsis myosin XI responsible for cytoplasmic streaming.Scientific reports · 2022Article
- Discovery of ultrafast myosin, its amino acid sequence, and structural features.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Optical control of fast and processive engineered myosins in vitro and in living cells.Nature chemical biology · 2021Article
- Characterization of ancestral myosin XI from Marchantia polymorpha by heterologous expression in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2020Article
- Heterologous transformation ofPlant biotechnology (Tokyo, Japan) · 2020Article
- Functional Diversity of Class XI Myosins in Arabidopsis thaliana.Plant & cell physiology · 2018Article
- Kinesins and Myosins: Molecular Motors that Coordinate Cellular Functions in Plants.Annual review of plant biology · 2018Review
- Update on Myosin Motors: Molecular Mechanisms and Physiological Functions.Plant physiology · 2018Review
- The WASP-Arp2/3 complex signal cascade is involved in actin-dependent sperm nuclei migration during double fertilization in tobacco and maize.Scientific reports · 2017Article
- Multi-Scale Characean Experimental System: From Electrophysiology of Membrane Transporters to Cell-to-Cell Connectivity, Cytoplasmic Streaming and Auxin Metabolism.Frontiers in plant science · 2016Review
- Navigating the plant cell: intracellular transport logistics in the green kingdom.Molecular biology of the cell · 2015Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Most myosins have a positively charged loop 2 with a cluster of lysine residues that bind to the negatively charged N-terminal segment of actin. However, the net charge of loop 2 of very fast Chara myosin is zero and there is no lysine cluster in it. In contrast, Chara myosin has a highly positively charged loop 3. To elucidate the role of these unique surface loops of Chara myosin in its high velocity and high actin-activated ATPase activity, we have undertaken mutational analysis using recombinant Chara myosin motor domain. It was found that net positive charge in loop 3 affected V(max) and K(app) of actin activated ATPase activity, while it affected the velocity only slightly. The net positive charge in loop 2 affected K(app) and the velocity, although it did not affect V(max). Our results suggested that Chara myosin has evolved to have highly positively charged loop 3 for its high ATPase activity and have less positively charged loop 2 for its high velocity. Since high positive charge in loop 3 and low positive charge in loop 2 seem to be one of the reasons for Chara myosin's high velocity, we manipulated charge contents in loops 2 and 3 of Dictyostelium myosin (class II). Removing positive charge from loop 2 and adding positive charge to loop 3 of Dictyostelium myosin made its velocity higher than that of the wild type, suggesting that the charge strategy in loops 2 and 3 is widely applicable.
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