ArticleProceedings of the National Academy of Sciences of the United States of America2009
Posttranslational control of transcription factor FixK2, a key regulator for the Bradyrhizobium japonicum-soybean symbiosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- A Structure-Based Analysis of the Evolution of Transcription Factors of the FNR/CRP Family.Biomolecules · 2026Review
- HPlant, cell & environment · 2023Review
- Fine-Tuning Modulation of Oxidation-Mediated Posttranslational Control ofInternational journal of molecular sciences · 2022Article
- Redox Regulation in Diazotrophic Bacteria in Interaction with Plants.Antioxidants (Basel, Switzerland) · 2021Review
- Multiple sensors provide spatiotemporal oxygen regulation of gene expression in a Rhizobium-legume symbiosis.PLoS genetics · 2021Article
- Construction of Aerobic/Anaerobic-Substrate-Induced Gene Expression Procedure for Exploration of Metagenomes From Subseafloor Sediments.Frontiers in microbiology · 2021Article
- An Alkane Sulfonate Monooxygenase Is Required for Symbiotic Nitrogen Fixation byApplied and environmental microbiology · 2019Article
- An Integrated Systems Approach Unveils New Aspects of Microoxia-Mediated Regulation inFrontiers in microbiology · 2019Article
- Reassessing the Structure and Function Relationship of the OAntioxidants & redox signaling · 2018Review
- Functional Genomics Approaches to Studying Symbioses between Legumes and Nitrogen-Fixing Rhizobia.High-throughput · 2018Review
- Prediction of reversible disulfide based on features from local structural signatures.BMC genomics · 2017Article
- FixKFrontiers in microbiology · 2017Article
- Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens.Applied and environmental microbiology · 2016Article
- RNA-Seq Analysis of Differential Gene Expression Responding to Different Rhizobium Strains in Soybean (Glycine max) Roots.Frontiers in plant science · 2016Article
- Thiol-based redox signaling in the nitrogen-fixing symbiosis.Frontiers in plant science · 2013Review
- The structure of Bradyrhizobium japonicum transcription factor FixK2 unveils sites of DNA binding and oxidation.The Journal of biological chemistry · 2013Article
- Copper starvation-inducible protein for cytochrome oxidase biogenesis in Bradyrhizobium japonicum.The Journal of biological chemistry · 2012Article
- Nitrous oxide production and consumption: regulation of gene expression by gas-sensitive transcription factors.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Bacterial adaptation of respiration from oxic to microoxic and anoxic conditions: redox control.Antioxidants & redox signaling · 2012Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhizobial FixK-like proteins play essential roles in activating genes for endosymbiotic life in legume root nodules, such as genes for micro-oxic respiration. In the facultative soybean symbiont, Bradyrhizobium japonicum, the FixK(2) protein is the key player in a complex regulatory network. The fixK(2) gene itself is activated by the 2-component regulatory system FixLJ in response to a moderate decrease of the oxygen tension, and the FixK(2) protein distributes and amplifies this response to the level of approximately 200 target genes. Unlike other members of the cAMP receptor protein family, to which FixK(2) belongs, the FixK(2) protein does not appear to be modulated by small effector molecules. Here, we show that a critical, single cysteine residue (C183) near the DNA-binding domain of FixK(2) confers sensitivity to oxidizing agents and reactive oxygen species. Oxidation-dependent inactivation occurs not only in vitro, as shown with cell-free transcription assays, but also in vivo, as shown by microarray-assisted transcriptome analysis of the FixK(2) regulon. The oxidation mechanism may involve a reversible dimerization by intermolecular disulfide-bridge formation and a direct, irreversible oxidation at the cysteine thiol, depending on the oxidizing agent. Mutational exchange of C183 to alanine renders FixK(2) resistant to oxidation, yet allows full activity, shown again both in vitro and in vivo. We hypothesize that posttranslational modification by reactive oxygen species is a means to counterbalance the cellular pool of active FixK(2), which would otherwise fill unrestrictedly through FixLJ-dependent synthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.