ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2009
Preferred sites of exocytosis and endocytosis colocalize during high- but not lower-frequency stimulation in mouse motor nerve terminals.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Chloroquine Affects Presynaptic Membrane Retrieval in Diaphragm Neuromuscular Junctions of Old Mice.International journal of molecular sciences · 2024Article
- Electrophysiological effects of BDNF and TrkB signaling at type-identified diaphragm neuromuscular junctions.Journal of neurophysiology · 2023Article
- Article
- Zebrafish neuromuscular junction: The power of N.Neuroscience letters · 2019Review
- Nanoscale Organization of Vesicle Release at Central Synapses.Trends in neurosciences · 2019Review
- Presynaptic mechanisms controlling calcium-triggered transmitter release at the neuromuscular junction.Current opinion in physiology · 2018Article
- Impact of spatiotemporal calcium dynamics within presynaptic active zones on synaptic delay at the frog neuromuscular junction.Journal of neurophysiology · 2018Article
- There Is No Simple Model of the Plasma Membrane Organization.Frontiers in cell and developmental biology · 2016Review
- Different dynamin blockers interfere with distinct phases of synaptic endocytosis during stimulation in motoneurones.The Journal of physiology · 2015Article
- Synaptic vesicle recycling: steps and principles.The EMBO journal · 2014Review
- Cell adhesion defines the topology of endocytosis and signaling.The EMBO journal · 2014Article
- A noncanonical postsynaptic transport route for a GPCR belonging to the serotonin receptor family.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Live imaging of bulk endocytosis in frog motor nerve terminals using FM dyes.Journal of neurophysiology · 2011Article
- Active zones and the readily releasable pool of synaptic vesicles at the neuromuscular junction of the mouse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Frequency-dependent modes of synaptic vesicle endocytosis and exocytosis at adult mouse neuromuscular junctions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Vesicle pools: lessons from adrenal chromaffin cells.Frontiers in synaptic neuroscience · 2011Article
- Article
- Why does endocytosis in single cells care which side up?BioarchitectureArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The spatial relationship of exocytosis and endocytosis in motor nerve terminals has been explored, with varied results, mostly in fixed preparations and without direct information on the utilization of each exocytic site. We sought to determine these spatial properties in real time using synaptopHluorin (spH) and FM4-64. Earlier we showed that nerve stimulation elicits the appearance of spH fluorescence hot spots, which mark preferred sites of exocytosis. Here we show that nerve stimulation in the presence of the styryl dye FM4-64 evokes hot spots of FM4-64 fluorescence. Their size, density, and rate of appearance are similar to the spH hot spots, but their rate of disappearance after stimulation was much slower (t(1/2) approximately 9 min vs approximately 10 s for spH hot spots), consistent with FM4-64 spots identifying bulk endocytosis and subsequent slow intracellular dispersion of nascent vesicles. Simultaneous imaging of both fluorophores revealed a strong colocalization of spH and FM4-64 spots, but only during high (100 Hz) stimulation. At 40 Hz stimulation, exocytic and endocytic spots did not colocalize. Our results are consistent with the hypothesis that hot spots of endocytosis, possibly in the form of bulk uptake, occur at or very near highly active exocytic sites during high-frequency stimulation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.