ArticleJournal of virology2010
Membrane orientation of the human papillomavirus type 16 E5 oncoprotein.
Article in Journal of virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Functions of Viroporins in the Viral Life Cycle and Their Regulation of Host Cell Responses.Frontiers in immunology · 2022Review
- Unravelling the Immunomodulatory Effects of Viral Ion Channels, towards the Treatment of Disease.Viruses · 2021Review
- The scaffold protein IQGAP1 is crucial for extravasation and metastasis.Scientific reports · 2020Article
- Murine neuroblastoma cell lines developed by conditional reprogramming preserve heterogeneous phenotypes observed in vivo.Laboratory investigation; a journal of technical methods and pathology · 2020Article
- Characteristics and Functions of the Yip1 Domain Family (YIPF), Multi-Span Transmembrane Proteins Mainly Localized to the Golgi Apparatus.Frontiers in cell and developmental biology · 2019Review
- Infectious Bursal Disease Virus Hijacks Endosomal Membranes as the Scaffolding Structure for Viral Replication.Journal of virology · 2018Article
- Glycosylated Triterpenoids as Endosomal Escape Enhancers in Targeted Tumor Therapies.Biomedicines · 2017Review
- Conditional cell reprogramming involves non-canonical β-catenin activation and mTOR-mediated inactivation of Akt.PloS one · 2017Article
- Emerging From the Unknown: Structural and Functional Features of Agnoprotein of Polyomaviruses.Journal of cellular physiology · 2016Review
- Review
- Topography of the Human Papillomavirus Minor Capsid Protein L2 during Vesicular Trafficking of Infectious Entry.Journal of virology · 2015Article
- YIP1 family member 4 (YIPF4) is a novel cellular binding partner of the papillomavirus E5 proteins.Scientific reports · 2015Article
- Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization.Journal of visualized experiments : JoVE · 2015Article
- Exon 4-encoded sequence is a major determinant of cytotoxicity of apolipoprotein L1.American journal of physiology. Cell physiology · 2015Article
- Human papillomavirus molecular biology and disease association.Reviews in medical virology · 2015Review
- The E5 proteins.Virology · 2013Review
- HPV16 E5 peptide vaccine in treatment of cervical cancer in vitro and in vivo.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2013Article
- Conditionally reprogrammed cells represent a stem-like state of adult epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cancer associated human papillomaviruses.Current opinion in virology · 2012Review
- High-risk human papillomavirus E5 oncoprotein displays channel-forming activity sensitive to small-molecule inhibitors.Journal of virology · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The E5 protein of human papillomavirus type 16 is a small, hydrophobic protein that localizes predominantly to membranes of the endoplasmic reticulum (ER). To define the orientation of E5 in these membranes, we employed a differential, detergent permeabilization technique that makes use of the ability of low concentrations of digitonin to selectively permeabilize the plasma membrane and saponin to permeabilize all cellular membranes. We then generated a biologically active E5 protein that was epitope tagged at both its N and C termini and determined the accessibility of these termini to antibodies in the presence and absence of detergents. In both COS cells and human ectocervical cells, the C terminus of E5 was exposed to the cytoplasm, whereas the N terminus was restricted to the lumen of the ER. Finally, the deletion of the E5 third transmembrane domain (and terminal hydrophilic amino acids) resulted in a protein with its C terminus in the ER lumen. Taken together, these topology findings are compatible with a model of E5 being a 3-pass transmembrane protein and with studies demonstrating its C terminus interacting with cytoplasmic proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.