ArticleJournal of clinical microbiology2010
Application of rpoB and zinc protease gene for use in molecular discrimination of Fusobacterium nucleatum subspecies.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
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- Paired oral clinical specimens reveal the underlying ecology supporting the emergence of inflammophilic microbiome communities.Microbiology spectrum · 2026Article
- AI-2 production inJournal of bacteriology · 2026Article
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- Integrating ANI and phylogenies for re-evaluation of Fusobacterium taxonomy and disease associations.Nature communications · 2026Article
- Paired oral clinical specimens reveal the underlying ecology supporting the emergence of inflammophilic microbiome communities.bioRxiv : the preprint server for biology · 2026Article
- Taxonomy, carcinogenic mechanisms, and advanced molecular diagnosis of Fusobacterium nucleatum in colorectal cancer: from bench to clinical practice.Frontiers in immunology · 2026Review
- Addressing controversy inGut microbes · 2025Article
- A Modified Method Incorporating Multiplex PCR Reveals Fusobacterium Prevalence in Southern Chinese Population and Its Correlations in Cancers.Microbial biotechnology · 2025Article
- The rise and evolving role ofCurrent research in microbial sciences · 2025Review
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- Stratification of Fusobacterium nucleatum by local health status in the oral cavity defines its subspecies disease association.Cell host & microbe · 2024Article
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- The prevalence ofbioRxiv : the preprint server for biology · 2023Article
- Profiling Fusobacterium infection at high taxonomic resolution reveals lineage-specific correlations in colorectal cancer.Nature communications · 2022Article
- More Than Just a Periodontal Pathogen -the Research Progress onFrontiers in cellular and infection microbiology · 2022Review
- A newly developed PCR-based method revealed distinct Fusobacterium nucleatum subspecies infection patterns in colorectal cancer.Microbial biotechnology · 2021Article
- Article
Corrections and comments
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Authors and funding
24 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fusobacterium nucleatum is classified into five subspecies that inhabit the human oral cavity (F. nucleatum subsp. nucleatum, F. nucleatum subsp. polymorphum, F. nucleatum subsp. fusiforme, F. nucleatum subsp. vincentii, and F. nucleatum subsp. animalis) based on several phenotypic characteristics and DNA-DNA hybridization patterns. However, the methods for detecting or discriminating the clinical isolates of F. nucleatum at the subspecies levels are laborious, expensive, and time-consuming. Therefore, in this study, the nucleotide sequences of the RNA polymerase beta-subunit gene (rpoB) and zinc protease gene were analyzed to discriminate the subspecies of F. nucleatum. The partial sequences of rpoB (approximately 2,419 bp), the zinc protease gene (878 bp), and 16S rRNA genes (approximately 1,500 bp) of the type strains of five subspecies, 28 clinical isolates of F. nucleatum, and 10 strains of F. periodonticum (as a control group) were determined and analyzed. The phylogenetic data showed that the rpoB and zinc protease gene sequences clearly delineated the subspecies of F. nucleatum and provided higher resolution than the 16S rRNA gene sequences in this respect. According to the phylogenetic analysis of rpoB and the zinc protease gene, F. nucleatum subsp. vincentii and F. nucleatum subsp. fusiforme might be classified into a single subspecies. Five clinical isolates could be delineated as a new subspecies of F. nucleatum. The results suggest that rpoB and the zinc protease gene are efficient targets for the discrimination and taxonomic analysis of the subspecies of F. nucleatum.
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