ArticleJournal of clinical microbiology2010
Detection and identification of Ehrlichia species in blood by use of PCR and electrospray ionization mass spectrometry.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 77 citations in OpenAlex.
- Xenodiagnosis to detect Borrelia burgdorferi infection: a first-in-human study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014Trial
- Direct molecular detection and genotyping of Borrelia burgdorferi from whole blood of patients with early Lyme disease.PloS one · 2012Trial
- Contribution of Limited Molecular Testing to Low Ehrlichiosis Diagnosis in High Incidence Area, North Carolina, USA.Emerging infectious diseases · 2025Article
- A MALDI-TOF nucleic acid mass spectrometry assay for rapid detection of fiveFrontiers in cellular and infection microbiology · 2025Article
- Forty Years of Molecular Diagnostics for Infectious Diseases.Journal of clinical microbiology · 2022Review
- Tick-borne zoonoses and commonly used diagnostic methods in human and veterinary medicine.Parasitology research · 2021Review
- Article
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- Assessment of Risk Factors and Outcomes of Severe Ehrlichiosis Infection.JAMA network open · 2020Article
- Rapid diagnosis and comprehensive bacteria profiling of sepsis based on cell-free DNA.Journal of translational medicine · 2020Article
- Molecular Testing of Serial Blood Specimens from Patients with Early Lyme Disease during Treatment Reveals Changing Coinfection with Mixtures of Borrelia burgdorferi Genotypes.Antimicrobial agents and chemotherapy · 2019Article
- Observational
- Broad-range survey of vector-borne pathogens and tick host identification of Ixodes ricinus from Southern Czech Republic.FEMS microbiology ecology · 2017Article
- Next-generation sequencing diagnostics of bacteremia in septic patients.Genome medicine · 2016Article
- An embedded microretroreflector-based microfluidic immunoassay platform.Lab on a chip · 2016Article
- The IRIDICA BAC BSI Assay: Rapid, Sensitive and Culture-Independent Identification of Bacteria and Candida in Blood.PloS one · 2016Article
- Rapid Diagnosis of Infection in the Critically Ill, a Multicenter Study of Molecular Detection in Bloodstream Infections, Pneumonia, and Sterile Site Infections.Critical care medicine · 2015Observational
- Sterile intra-amniotic inflammation in asymptomatic patients with a sonographic short cervix: prevalence and clinical significance.The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians · 2015Article
- DNA Sequence Signatures for Rapid Detection of Six Target Bacterial Pathogens Using PCR Assays.Microbiology insights · 2015Article
- Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Rapid detection and identification of Ehrlichia species improves clinical outcome for patients suspected of ehrlichiosis. We describe an assay that employs multilocus PCR and electrospray ionization mass spectrometry (PCR/ESI-MS) to detect and identify Ehrlichia species directly from blood specimens. The results were compared to those of a colorimetric microtiter PCR enzyme immunoassay (PCR-EIA) used as a diagnostic assay. Among 213 whole-blood samples collected from patients who were clinically suspected of ehrlichiosis from 1 May to 1 August 2008 at Vanderbilt University Hospital, 40 were positive for an Ehrlichia species by PCR/ESI-MS, giving a positive rate of 18.8%. In comparison to the PCR-EIA, PCR/ESI-MS possessed a sensitivity, a specificity, and positive and negative predictive values of 95.0%, 98.8%, 95.0%, and 98.8%, respectively. The 38 specimens that were positive for Ehrlichia by both PCR/ESI-MS and the PCR-EIA were further characterized to the species level, with 100% agreement between the two assays. In addition, Rickettsia rickettsii was detected by PCR/ESI-MS from four specimens that were confirmed retrospectively by serology and PCR-EIA. In three specimens, the PCR/ESI-MS assay identified Pseudomonas aeruginosa, Neisseria meningitidis, and Staphylococcus aureus; these were confirmed by culture and/or clinical diagnosis as being clinically relevant. From specimen processing to result reporting, the PCR/ESI-MS assay can be completed within 6 h, providing another laboratory tool for the diagnosis of ehrlichiosis. Moreover, this system may provide rapid detection and identification of additional pathogens directly from blood specimens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.