ArticleMolecular biology of the cell2010
Myosin II is essential for the spatiotemporal organization of traction forces during cell motility.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- Measurement of cellular traction forces during confined migration.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Emergence of multiple collective motility modes in a physical model of cell chains.NPJ systems biology and applications · 2025Article
- Review
- Metabolic reprogramming in response to cell mechanics.Biology of the cell · 2023Review
- Deconstructing the role of myosin contractility in force fluctuations within focal adhesions.Biophysical journal · 2022Article
- Modeling cell protrusion predicts how myosin II and actin turnover affect adhesion-based signaling.Biophysical journal · 2022Article
- Cell Migration Driven by Self-Generated Integrin Ligand Gradient on Ligand-Labile Surfaces.Current biology : CB · 2020Article
- Three-Dimensional Monolayer Stress Microscopy.Biophysical journal · 2019Article
- Techniques to stimulate and interrogate cell-cell adhesion mechanics.Extreme Mechanics Letters · 2018Article
- Enucleated cells reveal differential roles of the nucleus in cell migration, polarity, and mechanotransduction.The Journal of cell biology · 2018Article
- Dissipation of contractile forces: the missing piece in cell mechanics.Molecular biology of the cell · 2017Article
- Mechanosensitive Adhesion Explains Stepping Motility in Amoeboid Cells.Biophysical journal · 2017Article
- Self-organized mechano-chemical dynamics in amoeboid locomotion ofJournal of physics D: Applied physics · 2017Article
- Two-Layer Elastographic 3-D Traction Force Microscopy.Scientific reports · 2017Article
- Cooperative cell motility during tandem locomotion of amoeboid cells.Molecular biology of the cell · 2016Article
- For whom the cells pull: Hydrogel and micropost devices for measuring traction forces.Methods (San Diego, Calif.) · 2016Review
- Contractile dynamics change before morphological cues during fluorescence [corrected] illumination.Scientific reports · 2015Article
- Regulation of microtubule dynamics by DIAPH3 influences amoeboid tumor cell mechanics and sensitivity to taxanes.Scientific reports · 2015Article
- Three-dimensional balance of cortical tension and axial contractility enables fast amoeboid migration.Biophysical journal · 2015Article
- Cytoskeletal forces during signaling activation in Jurkat T-cells.Molecular biology of the cell · 2015Article
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Authors and funding
5 authors.
Funding
Abstract
Amoeboid motility requires spatiotemporal coordination of biochemical pathways regulating force generation and consists of the quasi-periodic repetition of a motility cycle driven by actin polymerization and actomyosin contraction. Using new analytical tools and statistical methods, we provide, for the first time, a statistically significant quantification of the spatial distribution of the traction forces generated at each phase of the cycle (protrusion, contraction, retraction, and relaxation). We show that cells are constantly under tensional stress and that wild-type cells develop two opposing "pole" forces pulling the front and back toward the center whose strength is modulated up and down periodically in each cycle. We demonstrate that nonmuscular myosin II complex (MyoII) cross-linking and motor functions have different roles in controlling the spatiotemporal distribution of traction forces, the changes in cell shape, and the duration of all the phases. We show that the time required to complete each phase is dramatically increased in cells with altered MyoII motor function, demonstrating that it is required not only for contraction but also for protrusion. Concomitant loss of MyoII actin cross-linking leads to a force redistribution throughout the cell perimeter pulling inward toward the center. However, it does not reduce significantly the magnitude of the traction forces, uncovering a non-MyoII-mediated mechanism for the contractility of the cell.
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