ArticleAmerican journal of physiology. Renal physiology2010
Characterization of vasopressin-responsive collecting duct adenylyl cyclases in the mouse.
Article in American journal of physiology. Renal physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 35 citations in OpenAlex.
- Cyclic Adenosine Monophosphate Signaling in Chronic Kidney Disease: Molecular Targets and Therapeutic Potentials.International journal of molecular sciences · 2024Review
- Emerging therapies for autosomal dominant polycystic kidney disease with a focus on cAMP signaling.Frontiers in molecular biosciences · 2022Review
- Evaluation of morphological traits and physiological variables of several Chinese goat breeds and their crosses.Tropical animal health and production · 2021Article
- Doxycycline Changes the Transcriptome Profile of mIMCD3 Renal Epithelial Cells.Frontiers in physiology · 2021Article
- Stimulation of the Epithelial NaFrontiers in physiology · 2021Article
- Modulation of polycystic kidney disease by G-protein coupled receptors and cyclic AMP signaling.Cellular signalling · 2020Review
- Renomedullary Interstitial Cell Endothelin A Receptors Regulate BP and Renal Function.Journal of the American Society of Nephrology : JASN · 2020Article
- Goat Genomic Resources: The Search for Genes Associated with Its Economic Traits.International journal of genomics · 2020Review
- Role of calcium in adult onset polycystic kidney disease.Cellular signalling · 2019Article
- Nephron prorenin receptor deficiency alters renal medullary endothelin-1 and endothelin receptor expression.Physiological research · 2018Article
- Identification of selection signals by large-scale whole-genome resequencing of cashmere goats.Scientific reports · 2017Article
- Histone deacetylase 6 inhibition reduces cysts by decreasing cAMP and CaThe Journal of biological chemistry · 2017Article
- PGEAmerican journal of physiology. Renal physiology · 2017Article
- STIM1fl/fl Ksp-Cre Mouse has Impaired Renal Water Balance.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2016Article
- Glycogen synthase kinase 3α regulates urine concentrating mechanism in mice.American journal of physiology. Renal physiology · 2015Article
- Tolvaptan plus pasireotide shows enhanced efficacy in a PKD1 model.Journal of the American Society of Nephrology : JASN · 2015Article
- Expression of adrenergic and cholinergic receptors in murine renal intercalated cells.The Journal of veterinary medical science · 2014Article
- Differential dopamine receptor subtype regulation of adenylyl cyclases in lipid rafts in human embryonic kidney and renal proximal tubule cells.Cellular signalling · 2014Article
- Regulation of nephron water and electrolyte transport by adenylyl cyclases.American journal of physiology. Renal physiology · 2014Review
- Lack of an effect of collecting duct-specific deletion of adenylyl cyclase 3 on renal Na+ and water excretion or arterial pressure.American journal of physiology. Renal physiology · 2014Article
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4 authors at 1 institution in 1 country.
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Abstract
Little is known about collecting duct adenylyl cyclase (AC) isoforms or regulation in the mouse. We performed RT-PCR for AC isoforms 1-9 in microdissected cortical (CCD) and outer medullary (OMCD) and acutely isolated inner medullary (IMCD) collecting duct. All collecting duct regions contained AC3, AC4, and AC6 mRNA, while CCD and OMCD, but not IMCD, also contained AC5 mRNA. Acutely isolated IMCD expressed AC3, AC4, and AC6 proteins by Western blot analysis. The mIMCD3 cell line expressed AC2, AC3, AC4, AC5, and AC6 mRNA; M-1 CCD cells expressed AC2, 3, 4, and 6, while mpkCCD cell lines contained AC3, AC4, and AC6 mRNA. AVP stimulated cAMP accumulation in acutely isolated mouse IMCD; this was reduced by chelation of extracellular calcium (EGTA) and almost completely abolished by blockade of calmodulin (W-7). Blockade of calmodulin kinase with KN-93 or endoplasmic reticulum calcium ATPase (thapsigargin) also reduced the AVP response. A similar inhibitory effect of W-7, KN-93, and thapsigargin was seen on forskolin-stimulated cAMP content in acutely isolated mouse IMCD. These three agents had the same pattern of blockade of AVP- or forskolin-stimulated AC activity in acutely isolated rat IMCD. AVP responsiveness in primary cultures of mouse IMCD was also reduced by W-7, KN-93, and thapsigargin. Small interfering RNA (siRNA) designed to knock down AC3 or AC6 in primary cultured mouse IMCD significantly reduced AVP-stimulated cAMP accumulation. Together, these data are consistent with a role of AC3 and AC6 in the activation of mouse collecting duct by AVP.
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