ArticleThe Journal of biological chemistry2010
Thyrostimulin, but not thyroid-stimulating hormone (TSH), acts as a paracrine regulator to activate the TSH receptor in mammalian ovary.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 89 citations in OpenAlex.
- Identification of TSHB gene expression profile in Duolang sheep and its functional role in granulosa cells.BMC genomics · 2026Article
- Phosphodiesterase 8 regulation of excitation-contraction coupling as a target for cardiovascular disease: is it my turn now?American journal of physiology. Heart and circulatory physiology · 2026Review
- Reduced glycoprotein hormone β5 links male aging and testosterone decline to increased adiposity.The Journal of clinical investigation · 2026Article
- Molecular and Functional Insights into Thyrostimulin and Its Subunits-GPA2/GPB5.International journal of molecular sciences · 2025Review
- Microarray evidence that 8-cell human embryos express some hormone family members including oxytocin.Journal of assisted reproduction and genetics · 2024Article
- A genomic region associated with iteroparous spawning phenology is linked with age-at-maturity in female steelhead trout.Evolutionary applications · 2024Article
- Review
- Atypical pituitary hormone-target tissue axis.Frontiers of medicine · 2023Review
- Ancestral glycoprotein hormone-receptor pathway controls growth inFrontiers in endocrinology · 2023Article
- Malignant Clinical Course of "Proliferative" Ovarian Struma: Diagnostic Challenges and Treatment Pitfalls.Diagnostics (Basel, Switzerland) · 2022Article
- Investigating the role of glycoprotein hormone GPA2/GPB5 signaling in reproduction in adult femaleFrontiers in insect science · 2022Article
- Genes Encoding the Glycoprotein Hormone GPA2/GPB5 and the Receptor LGR1 in a Female Prawn.Frontiers in endocrinology · 2022Article
- Malignant Struma Ovarii with Concurrent Thyroid Cancer: Outcomes during and after Pregnancy.European thyroid journal · 2021Article
- Expression of Endogenous Putative TSH Binding Protein in Orbit.Current issues in molecular biology · 2021Article
- Unconventional Actions of Glycoprotein Hormone Subunits: A Comprehensive Review.Frontiers in endocrinology · 2021Review
- What happens after a blood meal? A transcriptome analysis of the main tissues involved in egg production in Rhodnius prolixus, an insect vector of Chagas disease.PLoS neglected tropical diseases · 2020Article
- The Molecular Function and Clinical Role of Thyroid Stimulating Hormone Receptor in Cancer Cells.Cells · 2020Review
- VolcanoFinder: Genomic scans for adaptive introgression.PLoS genetics · 2020Article
- Thyroid Hormones and Functional Ovarian Reserve: Systemic vs. Peripheral Dysfunctions.Journal of clinical medicine · 2020Review
- A Novel Antigonadotropic Role of Thyroid Stimulating Hormone on Leydig Cell-Derived Mouse Leydig Tumor Cells-1 Line.Annals of the National Academy of Medical Sciences (India) · 2020Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The thyroid-stimulating hormone receptor (TSHR), activated by either TSH or the newly discovered glycoprotein hormone thyrostimulin, plays a central role in the control of body metabolism. Interestingly, in addition to its thyroid expression, we discovered that the mRNA level of TSHR is periodically regulated in rat ovary by gonadotropins. Ovarian microdissection followed by real-time PCR analysis indicated that granulosa cells show the highest level of TSHR expression. Cultures of follicles and primary granulosa cells demonstrated that the level of TSHR is up-regulated and decreased by the gonadotropin-driven cAMP cascade and estradiol production, respectively. Furthermore, in contrast to the negligible expression of TSH in the ovary, we also found by real-time PCR and immunohistochemical analysis that thyrostimulin is expressed mainly in oocytes. Evolving before the appearance of gonadotropins, thyrostimulin is considered the most ancestral glycoprotein hormone. Therefore, the presence of thyrostimulin in the ovary suggests that it may have a primitive function in reproduction when it activates ovarian TSHR. Next, we generated recombinant thyrostimulin protein and characterized its non-covalent heterodimeric nature. Using purified recombinant thyrostimulin, we show that the human ovarian cell line NIH:OVCAR-3 also expresses endogenous and functional TSHR. Using cultured rat granulosa cells isolated from different ovarian stages, we found that treatments with thyrostimulin significantly increase cAMP production and the c-fos gene response in the presence of gonadotropins. Thus, this study demonstrates that oocyte-derived thyrostimulin and granulosa cell-expressed TSHR compose a novel paracrine system in the ovary, where the activity is tightly controlled by gonadotropins.
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