ArticleJournal of mammary gland biology and neoplasia2009
Adipose-depleted mammary epithelial cells and organoids.
Article in Journal of mammary gland biology and neoplasia, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Maternal exercise during lactation remodels obesity-associated mammary metabolism and milk fatty acids, enhancing offspring lipid oxidation.American journal of physiology. Endocrinology and metabolism · 2026Article
- Implantation of engineered adipocytes suppresses tumor progression in cancer models.Nature biotechnology · 2025Article
- Reprogramming White Fat Cells for Adipose Manipulation Transplantation (AMT) Therapy.Bio-protocol · 2025Article
- Manganese-induced Precocious Puberty Alters Mammary Epithelial Cell Proliferation in Female Rats.Endocrinology · 2025Article
- Article
- Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications.Advanced drug delivery reviews · 2024Review
- Fatty Acid Metabolism Reprogramming in Advanced Prostate Cancer.Metabolites · 2021Review
- Developmental Expression of Claudins in the Mammary Gland.Journal of mammary gland biology and neoplasia · 2017Article
- Constitutive expression of microRNA-150 in mammary epithelium suppresses secretory activation and impairs de novo lipogenesis.Development (Cambridge, England) · 2016Article
- Thyroid hormone responsive protein Spot14 enhances catalysis of fatty acid synthase in lactating mammary epithelium.Journal of lipid research · 2014Article
- Liver x receptors stimulate lipogenesis in bovine mammary epithelial cell culture but do not appear to be involved in diet-induced milk fat depression in cows.Physiological reports · 2014Article
- The insulin receptor plays an important role in secretory differentiation in the mammary gland.American journal of physiology. Endocrinology and metabolism · 2013Article
- Obesity and overfeeding affecting both tumor and systemic metabolism activates the progesterone receptor to contribute to postmenopausal breast cancer.Cancer research · 2012Article
- Mammalian fatty acid synthase activity from crude tissue lysates tracing ¹³C-labeled substrates using gas chromatography-mass spectrometry.Analytical biochemistry · 2012Article
- Mammary adipocytes bioactivate 25-hydroxyvitamin D₃ and signal via vitamin D₃ receptor, modulating mammary epithelial cell growth.Journal of cellular biochemistry · 2011Article
- Prolactin-mediated regulation of lipid biosynthesis genes in vivo in the lactating mammary epithelial cell.American journal of physiology. Endocrinology and metabolism · 2011Article
- Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection.BMC cell biology · 2010Article
- Sterol regulatory element binding protein and dietary lipid regulation of fatty acid synthesis in the mammary epithelium.American journal of physiology. Endocrinology and metabolism · 2010Article
- Analysis of inhibitor of apoptosis protein family expression during mammary gland development.BMC developmental biology · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Analysis of genes and proteins involved in lipid biosynthesis in mammary epithelial cells (MECs) is complicated by the presence of adipose tissue in the mammary gland, which may be predominant in whole tissue lysates depending upon developmental stage. We have developed a method based on protocols used to establish primary mammary epithelial cell cultures that allows for analysis of MECs depleted of adipose. Adipose depletion yields enriched MECs that are suitable for gene expression profiling and protein analysis from a single mouse. Additionally, the phosphorylation of proteins is maintained, allowing investigation of signal transduction events. Application of this method to the analysis of MECs from genetically modified mice will aid in the identification of factors controlling tissue-specific events in the mammary gland. In contrast to other methods such as laser capture microdissection, the MEC enrichment method described here is performed using standard lab supplies, equipment, and techniques.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.