ArticleFrontiers in neural circuits2009
Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types.
Article in Frontiers in neural circuits, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- Optimized genetic tracers for viral mediated neuronal projection mapping.Cellular & molecular biology letters · 2026Article
- The Key Role of Somatostatin Neurons in the Suprachiasmatic Nucleus in Anxiety-Depression Comorbidity Following Sleep Deprivation.Molecular neurobiology · 2025Article
- Review
- Intramuscular Injection of rAAV2-retro for Low Motor Neuron Transduction: Evaluating Five Promoters.International journal of medical sciences · 2025Article
- Elevated vesicular Zn2+ in dorsal root ganglion neurons expressing the transporter TMEM163 causes age-associated itchy skin in mice.PLoS biology · 2024Article
- Local stimulation of pyramidal neurons in deep cortical layers of anesthetized rats enhances cortical visual information processing.Scientific reports · 2024Article
- Pharmacogenetic activation of parvalbumin interneurons in the prefrontal cortex rescues cognitive deficits induced by adolescent MK801 administration.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- Drug-Inducible Gene Therapy Effectively Reduces Spontaneous Seizures in Kindled Rats but Creates Off-Target Side Effects in Inhibitory Neurons.International journal of molecular sciences · 2023Article
- Preferential transduction of parvalbumin-expressing cortical neurons by AAV-mDLX5/6 vectors.Frontiers in neuroscience · 2023Article
- Advances in AAV technology for delivering genetically encoded cargo to the nonhuman primate nervous system.Current research in neurobiology · 2023Review
- Article
- Durability of transgene expression after rAAV gene therapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Review
- Developmental impact of glutamate transporter overexpression on dopaminergic neuron activity and stereotypic behavior.Molecular psychiatry · 2022Article
- Strategies for Targeting Neural Circuits: How to Manipulate Neurons Using Virus Vehicles.Frontiers in neural circuits · 2022Review
- Reelin restricts dendritic growth of interneurons in the neocortex.Development (Cambridge, England) · 2021Article
- Neocortical inhibitory interneuron subtypes are differentially attuned to synchrony- and rate-coded information.Communications biology · 2021Article
- Optogenetic Approach to Local Neuron Network Analysis of the Medullary Respiratory Center.Advances in experimental medicine and biology · 2021Article
- GABAergic Gene Regulatory Elements Used in Adeno-Associated Viral Vectors.Frontiers in neurology · 2021Review
- Viral tools for neuroscience.Nature reviews. Neuroscience · 2020Review
- Neural circuit analysis using a novel intersectional split intein-mediated split-Cre recombinase system.Molecular brain · 2020Article
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Authors and funding
7 authors.
Funding
Abstract
Short cell-type specific promoter sequences are important for targeted gene therapy and studies of brain circuitry. We report on the ability of short promoter sequences to drive fluorescent protein expression in specific types of mammalian cortical inhibitory neurons using adeno-associated virus (AAV) and lentivirus (LV) vectors. We tested many gene regulatory sequences derived from fugu (Takifugu rubripes), mouse, human, and synthetic composite regulatory elements. All fugu compact promoters expressed in mouse cortex, with only the somatostatin (SST) and the neuropeptide Y (NPY) promoters largely restricting expression to GABAergic neurons. However these promoters did not control expression in inhibitory cells in a subtype specific manner. We also tested mammalian promoter sequences derived from genes putatively coexpressed or coregulated within three major inhibitory interneuron classes (PV, SST, VIP). In contrast to the fugu promoters, many of the mammalian sequences failed to express, and only the promoter from gene A930038C07Rik conferred restricted expression, although as in the case of the fugu sequences, this too was not inhibitory neuron subtype specific. Lastly and more promisingly, a synthetic sequence consisting of a composite regulatory element assembled with PAX6 E1.1 binding sites, NRSE and a minimal CMV promoter showed markedly restricted expression to a small subset of mostly inhibitory neurons, but whose commonalities are unknown.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.