ArticlePrion
In vitro amplification of prions from milk in the detection of subclinical infections.
Article in Prion. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- Detection of Pathognomonic Biomarker PrPBiomolecules · 2020Review
- Article
- Differentiating ovine BSE from CH1641 scrapie by serial protein misfolding cyclic amplification.Molecular biotechnology · 2012Article
- Detection of prions in the faeces of sheep naturally infected with classical scrapie.Veterinary research · 2011Article
- Sparse PrP(Sc) accumulation in the placentas of goats with naturally acquired scrapie.BMC veterinary research · 2011Article
- Article
- Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prions can be amplified by serial protein misfolding cyclic amplification (sPMCA) from the milk of a high proportion of apparently healthy, scrapie exposed sheep with PRNP genotypes not previously associated with high disease penetrance. These data strongly suggest the widespread presence of subclinical scrapie infections within scrapie-exposed flocks containing sheep with a range of susceptible PRNP genotypes. These data also lead to the hypothesis that similar subclinical disease states may be common for other animal and human prion diseases. Furthermore, the application of sPMCA to milk provides a method to detect such subclinical disease. Here, we describe the high level amplification of bovine spongiform encephalopathy (BSE) prions from both ovine and bovine origin, a methodology that will facilitate the detection of any prions secreted within bovine and ovine milk during subclinical and clinical BSE disease.
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Registered trials
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