ArticleProceedings of the National Academy of Sciences of the United States of America2009
The endogenous siRNA pathway is involved in heterochromatin formation in Drosophila.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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85 citing papers in PubMed, 146 citations in OpenAlex.
- Evolution of siRNA Therapeutics: From Mechanistic Foundations to Clinical Expansion.Pharmaceutics · 2026Review
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- Limited impact of the siRNA pathway on transposable element expression in Aedes aegypti.BMC biology · 2025Article
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- Topoisomerase 3b facilitates piRNA biogenesis to promote transposon silencing and germ cell development.Cell reports · 2025Article
- An unnatural enzyme with endonuclease activity towards small non-coding RNAs.Nature communications · 2023Article
- Transposons and non-coding regions drive the intrafamily differences of genome size in insects.iScience · 2022Article
- Satellite DNAs in Health and Disease.Genes · 2022Review
- Mutational analysis of Aedes aegypti Dicer 2 provides insights into the biogenesis of antiviral exogenous small interfering RNAs.PLoS pathogens · 2022Article
- Satellite DNA-Mediated Gene Expression Regulation: Physiological and Evolutionary Implication.Progress in molecular and subcellular biology · 2021Article
- Drosophila Satellite Repeats at the Intersection of Chromatin, Gene Regulation and Evolution.Progress in molecular and subcellular biology · 2021Article
- Distinct Regulation of the Expression of Satellite DNAs in the BeetleInternational journal of molecular sciences · 2020Article
- Various modes of HP1a interactions with the euchromatic chromosome arms in Drosophila ovarian somatic cells.Chromosoma · 2020Article
- Active retrotransposons help maintain pericentromeric heterochromatin required for faithful cell division.Genome research · 2020Article
- Exploiting somatic piRNAs inLife science alliance · 2020Article
- Article
- Trafficking of siRNA precursors by the dsRBD protein Blanks in Drosophila.Nucleic acids research · 2020Article
- Article
- Article
- Nuclear Argonaute Piwi Gene Mutation Affects rRNA by Inducing rRNA Fragment Accumulation, Antisense Expression, and Defective Processing inInternational journal of molecular sciences · 2020Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new class of small RNAs (endo-siRNAs) produced from endogenous double-stranded RNA (dsRNA) precursors was recently shown to mediate transposable element (TE) silencing in the Drosophila soma. These endo-siRNAs might play a role in heterochromatin formation, as has been shown in S. pombe for siRNAs derived from repetitive sequences in chromosome pericentromeres. To address this possibility, we used the viral suppressors of RNA silencing B2 and P19. These proteins normally counteract the RNAi host defense by blocking the biogenesis or activity of virus-derived siRNAs. We hypothesized that both proteins would similarly block endo-siRNA processing or function, thereby revealing the contribution of endo-siRNA to heterochromatin formation. Accordingly, P19 as well as a nuclear form of P19 expressed in Drosophila somatic cells were found to sequester TE-derived siRNAs whereas B2 predominantly bound their longer precursors. Strikingly, B2 or the nuclear form of P19, but not P19, suppressed silencing of heterochromatin gene markers in adult flies, and altered histone H3-K9 methylation as well as chromosomal distribution of histone methyl transferase Su(var)3-9 and Heterochromatin Protein 1 in larvae. Similar effects were observed in dcr2, r2d2, and ago2 mutants. Our findings provide evidence that a nuclear pool of TE-derived endo-siRNAs is involved in heterochromatin formation in somatic tissues in Drosophila.
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