ArticleGenetics2010
FRQ-interacting RNA helicase mediates negative and positive feedback in the Neurospora circadian clock.
Article in Genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 95 citations in OpenAlex.
- Rhythmic nuclear import mediated by importins regulates the Neurospora circadian clock.Genetics · 2026Article
- Trends in circadian rhythms research in fungi since the millenium.Npj biological timing and sleep · 2026Review
- Core clock protein subcellular dynamics coordinate local and global circadian control in syncytia.The Journal of cell biology · 2026Article
- A daily cycle of White Collar Complex dephosphorylation sustains circadian rhythmicity inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Rhythmic Nuclear Import Mediated by Importins Regulates thebioRxiv : the preprint server for biology · 2026Article
- Casein kinase 1a mediates a two-step subunit remodeling mechanism to regulate the FRQ-FRH circadian clock complex.Nature communications · 2026Article
- Core Clock Protein Subcellular Dynamics Coordinate Local and Global Circadian Control in Syncytia.bioRxiv : the preprint server for biology · 2025Article
- Regulatory links between the circadian clock and stress-induced biomolecular condensates.Npj biological timing and sleep · 2025Review
- Disordered clock protein interactions and charge blocks turn an hourglass into a persistent circadian oscillator.Nature communications · 2024Article
- The Function, Regulation, and Mechanism of Protein Turnover in Circadian Systems inInternational journal of molecular sciences · 2024Review
- The Frq-Frh Complex Light-Dependently Delays Sfl1-Induced Microsclerotia Formation inJournal of fungi (Basel, Switzerland) · 2023Article
- A crucial role for dynamic expression of components encoding the negative arm of the circadian clock.Nature communications · 2023Article
- An Anatomy of Fungal Eye: Fungal Photoreceptors and Signalling Mechanisms.Journal of fungi (Basel, Switzerland) · 2023Review
- Conformational changes in the negative arm of the circadian clock correlate with dynamic interactomes involved in post-transcriptional regulation.Cell reports · 2023Article
- Functional analysis of 110 phosphorylation sites on the circadian clock protein FRQ identifies clusters determining period length and temperature compensation.G3 (Bethesda, Md.) · 2023Article
- Nutritional compensation of the circadian clock is a conserved process influenced by gene expression regulation and mRNA stability.PLoS biology · 2023Article
- Identification and expression of functionally conserved circadian clock genes in lichen-forming fungi.Scientific reports · 2022Article
- Data-driven modelling captures dynamics of the circadian clock of Neurospora crassa.PLoS computational biology · 2022Article
- The Essential and the Nonessential Roles of Four Clock Elements in the Circadian Rhythm ofJournal of fungi (Basel, Switzerland) · 2022Article
- Circadian Interactomics: How Research Into Protein-Protein Interactions Beyond the Core Clock Has Influenced the Model of Circadian Timekeeping.Journal of biological rhythms · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Neurospora circadian oscillator comprises FREQUENCY (FRQ) and its transcription activator, the White Collar Complex (WCC). Repression of WCC's transcriptional activity by FRQ via negative feedback is indispensable for clock function. An unbiased genetic screen that targeted mutants with defects in negative feedback regulation yielded a fully viable arrhythmic strain bearing a novel allele of FRQ-interacting RNA helicase (frh), an essential gene that encodes a putative exosome component protein. In the allele, frh(R806H), clock function is completely disturbed, while roles of FRQ-interacting RNA helicase (FRH) essential for viability are left intact. FRH(R806H) still interacts with FRQ, but interaction between the FRQ-FRH(R806H) complex (FFC) and WCC is severely affected. Phosphorylation of WC-1 is reduced in the mutant leading to constantly elevated WCC activity, which breaks the negative feedback loop. WCC levels are considerably reduced in the mutant, especially those of WC-1, consistent both with loss of positive feedback (FRQ-dependent WC-1 stabilization) and with a reduced level of the FRQ-mediated WCC phosphorylation that leads to high WCC activity accompanied by rapid transcription-associated turnover. FRH overexpression promotes WC-1 accumulation, confirming that FRH together with FRQ plays a role in WC-1 stabilization. Identification of a viable allele of frh, displaying virtually complete loss of both negative and positive circadian feedback, positions FRH as a core component of the central oscillator that is permissive for rhythmicity but appears not to modulate periodicity. Moreover, the results suggest that there are clock-specific roles for FRH that are distinct from the predicted essential exosome-associated functions for the protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.