ArticleApplied and environmental microbiology2010
Biosynthesis and degradation of H2O2 by vaginal lactobacilli.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 97 citations in OpenAlex.
- Genomic relatedness and clinical significance ofMicrobial genomics · 2021Trial
- Antimicrobial and inflammatory properties of South African clinical Lactobacillus isolates and vaginal probiotics.Scientific reports · 2019Trial
- Harnessing the Vaginal Microbiome: A New Frontier in the Prevention of Sexually Transmitted Infections.Expert reviews in molecular medicine · 2026Review
- Lactic Acid Bacteria-Derived Antimicrobial and Anti-Biofilm Strategies: Mechanisms, Functional Molecules, and Emerging Biomaterial Applications.International journal of molecular sciences · 2026Review
- The Vaginal Microbiome and Reproductive Health in Adolescents and Adults.Journal of pediatric and adolescent gynecology · 2025Review
- Antimicrobial activity of Limosilactobacillus fermentum strains isolated from the human oral cavity against Streptococcus mutans.Scientific reports · 2023Article
- Article
- Role of Genital Tract Bacteria in Promoting Endometrial Health in Cattle.Microorganisms · 2022Article
- Organic Acids Secreted byMolecules (Basel, Switzerland) · 2022Article
- In Vitro assessment of anti-Campylobacter activity of lactobacillus strains isolated from canine rectal swabs.BMC veterinary research · 2022Article
- Molecular mechanisms of the disturbance caused by malondialdehyde on probiotic Lactobacillus reuteri PL503.Microbial biotechnology · 2022Article
- The female reproductive tract microbiotas, inflammation, and gynecological conditions.Frontiers in reproductive health · 2022Review
- Lactobacilli metabolites restore E-cadherin and suppress MMP9 in cervical cancer cells.Current research in toxicology · 2022Article
- Optimized UV-Spectrophotometric Assay to Screen Bacterial Uricase Activity Using Whole Cell Suspension.Frontiers in microbiology · 2022Article
- Role of milk and honey in the tolerance of lactobacilli to oxidative stress.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Dual Role of Hydrogen Peroxide as an Oxidant in Pneumococcal Pneumonia.Antioxidants & redox signaling · 2021Review
- Article
- Characterization of the vaginal microbiome during cytolytic vaginosis using high-throughput sequencing.Journal of clinical laboratory analysis · 2019Article
- Antitumor mechanisms of bifidobacteria.Oncology letters · 2018Review
- Hydrogen peroxide production by lactobacilli promotes epithelial restitution during colitis.Redox biology · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrogen peroxide production by vaginal lactobacilli represents one of the most important defense mechanisms against vaginal colonization by undesirable microorganisms. To quantify the ability of a collection of 45 vaginal Lactobacillus strains to generate H(2)O(2), we first compared three published colorimetric methods. It was found that the use of DA-64 as a substrate rendered the highest sensitivity, while tetramethyl-benzidine (TMB) maintained its linearity from nanomolar to millimolar H(2)O(2) concentrations. Generation of H(2)O(2) was found to be especially common and strong for L. jensenii strains, while it was variable among L. crispatus and L. gasseri strains. Biosynthesis of H(2)O(2) only occurred upon agitation of the cultures, but the H(2)O(2)-producing machinery was already present in them before aeration started. Calcium, magnesium, manganese, and zinc ions did not affect H(2)O(2) production, while Cu(2+) inhibited the growth of Lactobacillus jensenii CECT 4306, which was chosen as a model strain. Cultures with Fe(3+), hemin, and hemoglobin did not accumulate H(2)O(2). Fe(3+) activated an extracellular peroxidase that destroyed the H(2)O(2) being produced by the cultures. This protected the lactobacilli against its antimicrobial effect. The production of the enzyme appears to be constitutive, the Fe(3+) ions being a necessary cofactor of the reaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.