ArticleApplied and environmental microbiology2010
Contributions of Francisella tularensis subsp. novicida chitinases and Sec secretion system to biofilm formation on chitin.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 62 citations in OpenAlex.
- Characterization of carABpyrB operon and role of pyrE in Francisella novicida biofilm.MicrobiologyOpen · 2026Article
- RelBE-mediated stress responses reshape cellular morphology ofMicrobiology spectrum · 2026Article
- Bacterial Acyl Carrier Proteins Are a Cytoplasmic Target for Different Cationic Antimicrobial and Antibiofilm Peptides.International journal of molecular sciences · 2026Article
- Characterization of proteins present in the biofilm matrix and outer membrane vesicles ofmBio · 2025Article
- Treatment of bacterial biothreat agents with a novel purified bioactive lactoferrin affects both growth and biofilm formation.Frontiers in cellular and infection microbiology · 2025Article
- Editorial: Reviews in microbial pathogenesis.Frontiers in microbiology · 2025Article
- An overview of viral chitinases: General properties and biotechnological potential.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- Bacterial Chitinases and Their Role in Human Infection.Infection and immunity · 2023Review
- Article
- Why vary what's working? Phase variation and biofilm formation inFrontiers in microbiology · 2022Review
- Eco-evolutionary feedbacks mediated by bacterial membrane vesicles.FEMS microbiology reviews · 2021Review
- Long-Term Survival of Virulent Tularemia Pathogens outside a Host in Conditions That Mimic Natural Aquatic Environments.Applied and environmental microbiology · 2021Article
- Chitin Degradation Machinery and Secondary Metabolite Profiles in the Marine BacteriumMarine drugs · 2021Article
- Phase Variation of LPS and Capsule Is Responsible for Stochastic Biofilm Formation inFrontiers in cellular and infection microbiology · 2021Article
- Development, Phenotypic Characterization and Genomic Analysis of aFrontiers in microbiology · 2021Article
- Genetic Determinants of Antibiotic Resistance inFrontiers in microbiology · 2021Review
- APathogens (Basel, Switzerland) · 2020Article
- The pathogenesis of cardiac microlesion formation during severe bacteremic infection.PLoS pathogens · 2020Review
- Mechanisms Affecting the Acquisition, Persistence and Transmission ofMicroorganisms · 2020Review
- Biofilm architecture: An emerging synthetic biology target.Synthetic and systems biotechnology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Francisella tularensis, the zoonotic cause of tularemia, can infect numerous mammals and other eukaryotes. Although studying F. tularensis pathogenesis is essential to comprehending disease, mammalian infection is just one step in the ecology of Francisella species. F. tularensis has been isolated from aquatic environments and arthropod vectors, environments in which chitin could serve as a potential carbon source and as a surface for attachment and growth. We show that F. tularensis subsp. novicida forms biofilms during the colonization of chitin surfaces. The ability of F. tularensis to persist using chitin as a sole carbon source is dependent on chitinases, since mutants lacking chiA or chiB are attenuated for chitin colonization and biofilm formation in the absence of exogenous sugar. A genetic screen for biofilm mutants identified the Sec translocon export pathway and 14 secreted proteins. We show that these genes are important for initial attachment during biofilm formation. We generated defined deletion mutants by targeting two chaperone genes (secB1 and secB2) involved in Sec-dependent secretion and four genes that encode putative secreted proteins. All of the mutants were deficient in attachment to polystyrene and chitin surfaces and for biofilm formation compared to wild-type F. novicida. In contrast, mutations in the Sec translocon and secreted factors did not affect virulence. Our data suggest that biofilm formation by F. tularensis promotes persistence on chitin surfaces. Further study of the interaction of F. tularensis with the chitin microenvironment may provide insight into the environmental survival and transmission mechanisms of this pathogen.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.