ArticleApplied and environmental microbiology2010
Lineage and host source are both correlated with levels of Shiga toxin 2 production by Escherichia coli O157:H7 strains.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Predicting clinical outcome ofMicrobial genomics · 2025Article
- CommensalAnimals : an open access journal from MDPI · 2023Article
- Pathogenomes and variations in Shiga toxin production among geographically distinct clones ofMicrobial genomics · 2022Article
- Variability in the Occupancy ofToxins · 2021Article
- Phylogeny, Prevalence, and Shiga Toxin (Stx) Production of Clinical Escherichia coli O157 Clade 2 Strains Isolated in Shimane Prefecture, Japan.Current microbiology · 2021Article
- Identification of Antibiotics That Diminish Disease in a Murine Model of Enterohemorrhagic Escherichia coli Infection.Antimicrobial agents and chemotherapy · 2020Article
- LysR-type transcriptional regulator OvrB encoded in O island 9 drives enterohemorrhagicVirulence · 2019Article
- Genotypic Features of Clinical and BovineMicroorganisms · 2018Review
- Computational Analysis of Host-Pathogen Protein Interactions between Humans and Different Strains of EnterohemorrhagicFrontiers in cellular and infection microbiology · 2017Article
- Colonization of Beef Cattle by Shiga Toxin-Producing Escherichia coli during the First Year of Life: A Cohort Study.PloS one · 2016Article
- Diversity of Survival Patterns among Escherichia coli O157:H7 Genotypes Subjected to Food-Related Stress Conditions.Frontiers in microbiology · 2016Article
- The Shiga toxin 2 production level in enterohemorrhagic Escherichia coli O157:H7 is correlated with the subtypes of toxin-encoding phage.Scientific reports · 2015Article
- Coculture of Escherichia coli O157:H7 with a Nonpathogenic E. coli Strain Increases Toxin Production and Virulence in a Germfree Mouse Model.Infection and immunity · 2015Article
- Escherichia coli O157:H7 strains harbor at least three distinct sequence types of Shiga toxin 2a-converting phages.BMC genomics · 2015Article
- Multilocus genotypic characterization of Escherichia coli O157:H7 recovered from food sources.Epidemiology and infection · 2015Article
- Geographically distinct Escherichia coli O157 isolates differ by lineage, Shiga toxin genotype, and total shiga toxin production.Journal of clinical microbiology · 2015Article
- Molecular typing of Escherichia coli O157:H7 isolates from Swedish cattle and human cases: population dynamics and virulence.Journal of clinical microbiology · 2014Article
- Life on the outside: role of biofilms in environmental persistence of Shiga-toxin producing Escherichia coli.Frontiers in microbiology · 2014Review
- Multilocus genotype analysis of Escherichia coli O157 isolates from Australia and the United States provides evidence of geographic divergence.Applied and environmental microbiology · 2013Article
- Evolution of the Stx2-encoding prophage in persistent bovine Escherichia coli O157:H7 strains.Applied and environmental microbiology · 2013Article
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11 authors.
Funding
Abstract
Escherichia coli O157:H7 strains fall into three major genetic lineages that differ in their distribution among humans and cattle. Several recent studies have reported differences in the expression of virulence factors between E. coli O157:H7 strains from these two host species. In this study, we wished to determine if important virulence-associated "mobile genetic elements" such as Shiga toxin 2 (Stx2)-encoding prophage are lineage restricted or are host source related and acquired independently of the pathogen genotype. DNA sequencing of the stx(2) flanking region from a lineage II (LII) strain, EC970520, revealed that the transcriptional activator gene Q in LI strain EDL933 (upstream of stx(2)) is replaced by a pphA (serine/threonine phosphatase) homologue and an altered Q gene in this and all other LII strains tested. In addition, nearly all LI strains carried stx(2), whereas all LII strains carried variant stx(2c) and 4 of 14 LI/II strains had copies of both stx(2) and variant stx(2c). Real-time PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) demonstrated that LI and LI/II strains produce significantly more stx(2) mRNA and Stx2 than LII strains. However, among LI strains significantly more Stx2 is also produced by strains from humans than from cattle. Therefore, lineage-associated differences among E. coli O157:H7 strains such as prophage content, toxin type, and toxin expression may contribute to host isolation bias. However, the level of Stx2 production alone may also play an important role in the within-lineage association of E. coli O157:H7 strains with human clinical disease.
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