ArticleApplied and environmental microbiology2010
Rapid evaluation of the mycobactericidal efficacy of disinfectants in the quantitative carrier test EN 14563 by using fluorescent Mycobacterium terrae.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
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- The tuberculosis necrotizing toxin is an NADThe Journal of biological chemistry · 2019Article
- Role of theFrontiers in microbiology · 2018Article
- A Macrophage Infection Model to Predict Drug Efficacy Against Mycobacterium Tuberculosis.Assay and drug development technologies · 2016Article
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- Mycobacterium marinum mmar_2318 and mmar_2319 are Responsible for Lipooligosaccharide Biosynthesis and Virulence Toward Dictyostelium.Frontiers in microbiology · 2015Article
- Evaluation of mycobactericidal activity of selected chemical disinfectants and antiseptics according to European standards.Medical science monitor : international medical journal of experimental and clinical research · 2014Article
- A multicopper oxidase is required for copper resistance in Mycobacterium tuberculosis.Journal of bacteriology · 2013Article
- Effect of different energy levels of microwave on disinfection of dental stone casts.Journal of dental research, dental clinics, dental prospects · 2013Article
- An accurate method for the qualitative detection and quantification of mycobacterial promoter activity.The open microbiology journal · 2013Article
- Illegitimate recombination: an efficient method for random mutagenesis in Mycobacterium avium subsp. hominissuis.BMC microbiology · 2012Article
- Highly fluorescent GFPm 2+ -based genome integration-proficient promoter probe vector to study Mycobacterium tuberculosis promoters in infected macrophages.Microbial biotechnology · 2012Article
- Mycobacterium tuberculosis expresses ftsE gene through multiple transcripts.Current microbiology · 2011Article
- The ftsZ Gene of Mycobacterium smegmatis is expressed Through Multiple Transcripts.The open microbiology journal · 2011Article
- Role of porins in iron uptake by Mycobacterium smegmatis.Journal of bacteriology · 2010Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To prevent transmission of mycobacterial pathogens, medical devices must be disinfected by germicides with proven mycobactericidal activity. The quantitative carrier test EN 14563 provides an international standard for evaluation of the mycobactericidal activity of disinfectants under practical conditions. However, tests according to the EN 14563 standard are based on cultivation, and results are available only after 21 days. The aim of this study was to accelerate assessment of dosage and contact times of mycobactericidal preparations based on the EN 14563 standard. To this end, a gfp gene was constructed with a codon usage adapted for Mycobacterium tuberculosis. Expression of the gfp(m)(2+) gene in Mycobacterium terrae improved the detection sensitivity by 10-fold over that with a previously used reporter strain. Peracetic acid and a cation-active formulation were tested as commercially available disinfectants for medical devices. M. terrae expressing gfp(m)(2+) was used to determine dosage and contact times for the two test germicides. Fluorescence measurements correlated well with growth of the reporter strain, demonstrating that the fluorescence reliably indicated the number of viable cells. The fluorescence enabled us to determine the mycobactericidal efficacy of the test disinfectants according to the quantitative carrier test EN 14563 standard within at least 15 days. In conclusion, this study establishes gfp(m)(2+)-expressing M. terrae as a new reporter strain for reliable evaluation of mycobactericidal activities of disinfectants with a superior sensitivity and in a significantly shorter time than previously possible.
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