Evidence map›Paper›PMID 19948854›Full record

ArticleApplied and environmental microbiology2010

Use of green fluorescent protein to monitor cell envelope stress in Lactococcus lactis.

Ana Belén Campelo, Ana Rodríguez, Beatriz Martínez

Open access · greenAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Ana Belén CampeloDepartment of Technology and Biotechnology of Dairy Products, Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Apdo. 85, 33300 Villaviciosa, Asturias, Spain.
Ana Rodríguez
Beatriz Martínez
Instituto de Productos Lácteos de Asturias · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A Lactococcus lactis reporter system suitable to detect cell envelope stress in high-throughput settings was developed by fusing the CesR-regulated promoter of llmg0169 to the gfp(uv) gene. A dot blot assay allowed fast detection of green fluorescent protein (GFP) fluorescence even at low production levels. Unexpectedly, this promoter was also induced by mitomycin C via CesR.

Indexed as

Bacterial ProteinsCell MembraneCell WallCloning, MolecularDNA, BacterialFermentationFluorescent DyesGene Expression Regulation, BacterialGenes, BacterialGenetic VectorsGreen Fluorescent ProteinsIndustrial MicrobiologyLactococcus lactisMicrobial ViabilityMicroscopy, FluorescencePromoter Regions, GeneticBacterial ProteinsDNA, BacterialFluorescent DyesGreen Fluorescent ProteinsRecombinant Fusion Proteins

Identifiers

PMID19948854
PMCPMC2813010
OpenAlexW2091429575

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.