ArticleReproduction (Cambridge, England)2010
Aquaporin 9 expression in the developing rat epididymis is modulated by steroid hormones.
Article in Reproduction (Cambridge, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Aquaporins in Reproductive System.Advances in experimental medicine and biology · 2023Review
- CFTR modulates aquaporin-mediated glycerol permeability in mouse Sertoli cells.Cellular and molecular life sciences : CMLS · 2022Article
- The Multifaceted Role of Aquaporin-9 in Health and Its Potential as a Clinical Biomarker.Biomolecules · 2022Review
- Review
- Aquaglyceroporin Modulators as Emergent Pharmacological Molecules for Human Diseases.Frontiers in molecular biosciences · 2022Review
- Recent Update on the Molecular Mechanisms of Gonadal Steroids Action in Adipose Tissue.International journal of molecular sciences · 2021Review
- Hormonal control of vas deferens fluid volume and aquaporin expression in rats.Journal of molecular histology · 2019Article
- Estrogen Modulates Glycerol Permeability in Sertoli Cells through Downregulation of Aquaporin-9.Cells · 2018Article
- The cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost.Journal of anatomy · 2018Article
- Sex-dependent expression of water channel AQP1 along the rat nephron.American journal of physiology. Renal physiology · 2015Article
- CFTR interacts with ZO-1 to regulate tight junction assembly and epithelial differentiation through the ZONAB pathway.Journal of cell science · 2014Article
- Reduced hepatic aquaporin-9 and glycerol permeability are related to insulin resistance in non-alcoholic fatty liver disease.International journal of obesity (2005) · 2014Article
- Plasticity of basal cells during postnatal development in the rat epididymis.Reproduction (Cambridge, England) · 2013Article
- cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit.Developmental biology · 2012Article
- Pathways of arsenic uptake and efflux.Current topics in membranes · 2012Article
- Estrogen, efferent ductules, and the epididymis.Biology of reproduction · 2011Review
- Estrogens and development of the rete testis, efferent ductules, epididymis and vas deferens.Differentiation; research in biological diversityArticle
- Ontogeny of estrogen receptors in human male and female fetal reproductive tracts.Differentiation; research in biological diversityReview
- Disruption of estrogen receptor signaling and similar pathways in the efferent ductules and initial segment of the epididymis.SpermatogenesisReview
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Fluid and solute transport across the epithelium of the male excurrent duct is important for sperm maturation and storage. Aquaporin 9 (AQP9), which allows permeation of water and neutral solutes, is abundant throughout the male reproductive tract, where it is expressed at the apical membrane of rat epididymal principal cells as early as at 1 week of age. We evaluated the effect of neonatal exposure to: 1) a GNRH antagonist (GNRHa); 2) diethylstilbestrol (DES); 3) ethinyl estradiol (EE); 4) DES plus testosterone (DES+TE); and 5) the anti-androgen flutamide on AQP9 expression in the epididymis of peripubertal rats. Control groups received the vehicle alone. In 25-day-old rats, quantification of the mean pixel intensity of immunofluorescence-stained sections showed a significant decrease in AQP9 staining in the apical membrane of epididymal principal cells after treatments with GNRHa, DES, or flutamide, compared to controls. These results were confirmed by western blotting. While EE induced a marked decrease in AQP9 levels by western blotting, the decrease in AQP9-associated fluorescence was not significant compared to controls. DES+TE-treated rats showed levels of AQP9 protein similar to controls, indicating maintenance of AQP9 expression by testosterone treatment in the presence of DES. Our data show that expression of AQP9 in the developing rat epididymis is downregulated by neonatal DES, GNRHa, EE, and flutamide, and that the effects mediated by estrogens can be prevented by testosterone administration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.