ArticleThe Journal of molecular diagnostics : JMD2010
A one-step, real-time PCR assay for rapid detection of rhinovirus.
Article in The Journal of molecular diagnostics : JMD, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01376830 (Biomarkers and Clinical Characteristics That Identify the Risk of an Exacerbation Following Viral Upper Respiratory Tract Infections in COPD Patients), which is not on this map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Biomarkers and Clinical Characteristics That Identify the Risk of an Exacerbation Following Viral Upper Respiratory Tract Infections in COPD Patients
Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Comparative Investigation and Trends of Respiratory Viruses Using Wastewater-Based Epidemiological Surveillance in Patras, Greece.Food and environmental virology · 2026Article
- hMPV co-infections: Distinct immunopathogenic mechanisms and clinical implications of viral and bacterial pathogenesis.Folia microbiologica · 2025Review
- Human metapneumovirus (hMPV) in 2025: emerging trends and insights from community and hospital-based respiratory panel analyses-a comprehensive review.Virology journal · 2025Review
- Detection of Rhinovirus in Clinical Samples by PCR.Methods in molecular biology (Clifton, N.J.) · 2025Article
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- Changes in virus detection in hospitalized children before and after the severe acute respiratory syndrome coronavirus 2 pandemic.Influenza and other respiratory viruses · 2022Article
- Nearly Complete Genome Sequences of 12 Types of Human Rhinoviruses Isolated from Pediatric Inpatients in Fukushima, Japan.Microbiology resource announcements · 2022Article
- Epidemiological and clinical characteristics of infections with seasonal human coronavirus and respiratory syncytial virus in hospitalized children immediately before the coronavirus disease 2019 pandemic.Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2022Article
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- Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq.Nature communications · 2021Article
- Microfluidic-based virus detection methods for respiratory diseases.Emergent materials · 2021Review
- Recent advances in the detection of respiratory virus infection in humans.Journal of medical virology · 2020Review
- Development of real-time fluorescent reverse transcription loop-mediated isothermal amplification assays for rhinovirus detection.Journal of medical virology · 2019Article
- Development of real-time fluorescent reverse transcription loop-mediated isothermal amplification assay with quenching primer for influenza virus and respiratory syncytial virus.Journal of virological methods · 2019Article
- Propagation of Rhinovirus C in Differentiated Immortalized Human Airway HBEC3-KT Epithelial Cells.Viruses · 2019Article
- A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus.Virology journal · 2018Article
- Development of fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) using quenching probes for the detection of the Middle East respiratory syndrome coronavirus.Journal of virological methods · 2018Article
- Performance of a Taqman Assay for Improved Detection and Quantification of Human Rhinovirus Viral Load.Scientific reports · 2016Article
- Critical analysis of rhinovirus RNA load quantification by real-time reverse transcription-PCR.Journal of clinical microbiology · 2012Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One-step, real-time PCR assays for rhinovirus have been developed for a limited number of PCR amplification platforms and chemistries, and some exhibit cross-reactivity with genetically similar enteroviruses. We developed a one-step, real-time PCR assay for rhinovirus by using a sequence detection system (Applied Biosystems; Foster City, CA). The primers were designed to amplify a 120-base target in the noncoding region of picornavirus RNA, and a TaqMan (Applied Biosystems) degenerate probe was designed for the specific detection of rhinovirus amplicons. The PCR assay had no cross-reactivity with a panel of 76 nontarget nucleic acids, which included RNAs from 43 enterovirus strains. Excellent lower limits of detection relative to viral culture were observed for the PCR assay by using 38 of 40 rhinovirus reference strains representing different serotypes, which could reproducibly detect rhinovirus serotype 2 in viral transport medium containing 10 to 10,000 TCID(50) (50% tissue culture infectious dose endpoint) units/ml of the virus. However, for rhinovirus serotypes 59 and 69, the PCR assay was less sensitive than culture. Testing of 48 clinical specimens from children with cold-like illnesses for rhinovirus by the PCR and culture assays yielded detection rates of 16.7% and 6.3%, respectively. For a batch of 10 specimens, the entire assay was completed in 4.5 hours. This real-time PCR assay enables detection of many rhinovirus serotypes with the Applied Biosystems reagent-instrument platform.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.