ArticleGenes & development2009
Quantitative proteomic analysis of purified yeast kinetochores identifies a PP1 regulatory subunit.
Article in Genes & development, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 80 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
80 citing papers in PubMed, 109 citations in OpenAlex.
- An interdependent Cbf1-CCAN interaction stabilizes the budding yeast kinetochore.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cdc5-mediated Ulp2 phosphorylation controls the timing of polySUMOylation during the cell cycle.The Journal of cell biology · 2026Article
- An interdependent Cbf1-CCAN interaction stabilizes the budding yeast kinetochore.bioRxiv : the preprint server for biology · 2026Article
- Coupling Proximity Biotinylation with Genomic Targeting to Characterize Locus-Specific Changes in Chromatin Environments.Journal of proteome research · 2025Article
- Evidence of 14-3-3 proteins contributing to kinetochore integrity and chromosome congression during mitosis.Journal of cell science · 2024Article
- Kinetochore-microtubule error correction for biorientation: lessons from yeast.Biochemical Society transactions · 2024Review
- Higher-order protein assembly controls kinetochore formation.Nature cell biology · 2024Article
- A new layer of regulation of chromosomal passenger complex (CPC) translocation in budding yeast.Molecular biology of the cell · 2023Article
- Review
- Yeast Fin1-PP1 dephosphorylates an Ipl1 substrate, Ndc80, to remove Bub1-Bub3 checkpoint proteins from the kinetochore during anaphase.PLoS genetics · 2021Article
- Cell-cycle phospho-regulation of the kinetochore.Current genetics · 2021Review
- The Proteomic Landscape of Centromeric Chromatin Reveals an Essential Role for the Ctf19Current biology : CB · 2021Article
- Article
- The Opposing Functions of Protein Kinases and Phosphatases in Chromosome Bipolar Attachment.International journal of molecular sciences · 2019Review
- The molecular basis of monopolin recruitment to the kinetochore.Chromosoma · 2019Article
- The stoichiometry of the outer kinetochore is modulated by microtubule-proximal regulatory factors.The Journal of cell biology · 2019Article
- A Kinesin-5, Cin8, Recruits Protein Phosphatase 1 to Kinetochores and Regulates Chromosome Segregation.Current biology : CB · 2018Article
- Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast.Scientific reports · 2018Article
- Kinase and Phosphatase Cross-Talk at the Kinetochore.Frontiers in cell and developmental biology · 2018Review
- Dynamic localization of a yeast development-specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation.Molecular biology of the cell · 2017Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The kinetochore is a macromolecular complex that controls chromosome segregation and cell cycle progression. When sister kinetochores make bioriented attachments to microtubules from opposite poles, the spindle checkpoint is silenced. Biorientation and the spindle checkpoint are regulated by a balance between the Ipl1/Aurora B protein kinase and the opposing activity of protein phosphatase I (PP1). However, little is known about the regulation of PP1 localization and activity at the kinetochore. Here, we developed a method to purify centromere-bound kinetochores and used quantitative proteomics to identify the Fin1 protein as a PP1 regulatory subunit. The Fin1/PP1 complex is regulated by phosphorylation and 14-3-3 protein binding. When Fin1 is mislocalized, bipolar spindles fail to assemble but the spindle checkpoint is inappropriately silenced due to PP1 activity. These data suggest that Fin1 is a PP1 regulatory subunit whose spatial and temporal activity must be precisely controlled to ensure genomic stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.