ArticleThe Journal of biological chemistry2010
Ubiquitin ligase components Cullin4 and DDB1 are essential for DNA methylation in Neurospora crassa.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 64 citations in OpenAlex.
- Paf1C regulates the Neurospora circadian clock by promoting the transcription elongation of frequency.PLoS genetics · 2025Article
- SET-1-mediated H3K4me3 modification regulates catalase-3 expression in Neurospora crassa.Current genetics · 2025Article
- Transcription factor VIB-1 activates catalase-3 expression by promoting PIC assembly in Neurospora crassa.Nucleic acids research · 2025Article
- Review
- H3T11 phosphorylation by CKII is required for heterochromatin formation in Neurospora.Nucleic acids research · 2024Article
- Sensing of H2O2-induced oxidative stress by the UPF factor complex is crucial for activation of catalase-3 expression in Neurospora.PLoS genetics · 2023Article
- Genetic requirements for repair of lesions caused by single genomic ribonucleotides in S phase.Nature communications · 2023Article
- Article
- A role for the mitotic proteins Bub3 and BuGZ in transcriptional regulation of catalase-3 expression.PLoS genetics · 2022Article
- H3K56 deacetylation and H2A.Z deposition are required for aberrant heterochromatin spreading.Nucleic acids research · 2022Article
- The histone H3K9M mutation synergizes with H3K14 ubiquitylation to selectively sequester histone H3K9 methyltransferase Clr4 at heterochromatin.Cell reports · 2021Article
- F-box protein CFK1 interacts with and degrades de novo DNA methyltransferase in Arabidopsis.The New phytologist · 2021Article
- NC2 complex is a key factor for the activation of catalase-3 transcription by regulating H2A.Z deposition.Nucleic acids research · 2020Article
- Two dominant selectable markers for genetic manipulation in Neurospora crassa.Current genetics · 2020Article
- The Neurospora RNA polymerase II kinase CTK negatively regulates catalase expression in a chromatin context-dependent manner.Environmental microbiology · 2020Article
- Limited DNA Repair Gene Repertoire in Ascomycete Yeast Revealed by Comparative Genomics.Genome biology and evolution · 2019Article
- Transcription factor CBF-1 is critical for circadian gene expression by modulating WHITE COLLAR complex recruitment to the frq locus.PLoS genetics · 2018Article
- Increasing the Unneddylated Cullin1 Portion Rescues theMolecular and cellular biology · 2017Article
- Up-Frameshift Protein UPF1 RegulatesGenetics · 2017Article
- Antisense transcription licenses nascent transcripts to mediate transcriptional gene silencing.Genes & development · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation and H3K9 trimethylation are involved in gene silencing and heterochromatin assembly in mammals and fungi. In the filamentous fungus Neurospora crassa, it has been demonstrated that H3K9 trimethylation catalyzed by histone methyltransferase DIM-5 is essential for DNA methylation. Trimethylated H3K9 is recognized by HP1, which then recruits the DNA methyltransferase DIM-2 to methylate the DNA. Here, we show that in Neurospora, ubiquitin ligase components Cullin4 and DDB1 are essential for DNA methylation. These proteins regulate DNA methylation through their effects on the trimethylation of histone H3K9. In addition, we showed that the E3 ligase activity of the Cul4-based ubiquitin ligase is required for its function in histone H3K9 trimethylation in Neurospora. Furthermore, we demonstrated that Cul4 and DDB1 are associated with the histone methyltransferase DIM-5 protein in vivo. Together, these results suggest a mechanism for DNA methylation control that may be applicable in other eukaryotic organisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.