ArticleThe Journal of biological chemistry2010
Long transcripts from dinoflagellate chloroplast minicircles suggest "rolling circle" transcription.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Interpreting the complexities of the plastid genome in dinoflagellates: a mini-review of recent advances.Plant molecular biology · 2024Review
- Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination.Nature communications · 2024Article
- DNA:RNA Hybrids Are Major Dinoflagellate Minicircle Molecular Types.International journal of molecular sciences · 2023Article
- Plastid Transcript Editing across Dinoflagellate Lineages Shows Lineage-Specific Application but Conserved Trends.Genome biology and evolution · 2018Article
- Transcripts in the Plasmodium Apicoplast Undergo Cleavage at tRNAs and Editing, and Include Antisense Sequences.Protist · 2016Article
- Gene Expression Variation Resolves Species and Individual Strains among Coral-Associated Dinoflagellates within the Genus Symbiodinium.Genome biology and evolution · 2016Article
- Diversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga Karenia mikimotoi.Plant molecular biology · 2016Article
- Massive gene transfer and extensive RNA editing of a symbiotic dinoflagellate plastid genome.Genome biology and evolution · 2014Article
- Evolution of chloroplast transcript processing in Plasmodium and its chromerid algal relatives.PLoS genetics · 2014Article
- Functional remodeling of RNA processing in replacement chloroplasts by pathways retained from their predecessors.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Polyuridylylation and processing of transcripts from multiple gene minicircles in chloroplasts of the dinoflagellate Amphidinium carterae.Plant molecular biology · 2012Article
- Transcriptional response of two core photosystem genes in Symbiodinium spp. exposed to thermal stress.PloS one · 2012Article
- Article
- Transcription of the apicoplast genome.Molecular and biochemical parasitologyReview
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chloroplast genome of a dinoflagellate consists of a group of small circular DNA molecules (minicircles), most of which carry a single gene. With RT-PCR, primer extension, and Northern analyses, we show that the entire minicircle is transcribed and that some minicircles can produce RNAs larger than themselves. Using an RNA ligase-mediated rapid amplification of cDNA ends method, we were able to detect large processed precursors that are generated by endonucleolytic cleavage of an even longer molecule. This cleavage produces the mature mRNA 3'-end and at the same time the 5'-end of the precursor. The tRNAs encoded by the petD and psbE minicircles appear to be processed in the same way. We propose a "rolling circle" model for chloroplast transcription in which transcription would proceed continuously around the minicircular DNA to produce transcripts larger than the minicircle itself. These transcripts would be further processed into discrete mature mRNAs and tRNAs.
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