ArticleThe Journal of physiology2010
Modulation of the cold-activated cation channel TRPM8 by surface charge screening.
Article in The Journal of physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
- Direct modulation of TRPM8 ion channels by rapamycin and analog macrolide immunosuppressants.eLife · 2025Article
- The Role of TRP Channels in the Metastatic Cascade.Pharmaceuticals (Basel, Switzerland) · 2018Review
- Activation of Human Transient Receptor Potential Melastatin-8 (TRPM8) by Calcium-Rich Particulate Materials and Effects on Human Lung Cells.Molecular pharmacology · 2017Article
- Sex differences in mouse Transient Receptor Potential Cation Channel, Subfamily M, Member 8 expressing trigeminal ganglion neurons.PloS one · 2017Article
- New and notable ion-channels in the sarcoplasmic/endoplasmic reticulum: do they support the process of intracellular Ca²⁺ release?The Journal of physiology · 2015Review
- Regulation of the transient receptor potential channel TRPM3 by phosphoinositides.The Journal of general physiology · 2015Article
- Surface expression and channel function of TRPM8 are cooperatively controlled by transmembrane segments S3 and S4.Pflugers Archiv : European journal of physiology · 2013Article
- N-glycosylation of TRPM8 ion channels modulates temperature sensitivity of cold thermoreceptor neurons.The Journal of biological chemistry · 2012Article
- Ligand stoichiometry of the cold- and menthol-activated channel TRPM8.The Journal of physiology · 2011Article
- Characterization of TRPM8-like channels activated by the cooling agent icilin in the macrophage cell line RAW 264.7.The Journal of membrane biology · 2011Article
- Complex regulation of the TRPM8 cold receptor channel: role of arachidonic acid release following M3 muscarinic receptor stimulation.The Journal of biological chemistry · 2011Article
- Contribution of the S5-pore-S6 domain to the gating characteristics of the cation channels TRPM2 and TRPM8.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
TRPM8, a cation channel activated by cold and by cooling agents such as menthol and icilin, is critically involved in somatosensory cold sensation. Ion fluxes through TRPM8 are highly sensitive to changes in extracellular Ca(2+) and pH, but the mechanisms underlying this type of modulation are poorly understood. Here we provide evidence that inhibition of TRPM8 currents by extracellular divalent cations and protons is due to surface charge screening. We demonstrate that increasing concentrations of divalent cations or protons cause parallel shifts of the voltage dependence of TRPM8 activation towards positive potentials. These shifts were interpreted using the Gouy-Chapman-Stern theory, yielding an estimate for the density of fixed negative surface charge between 0.0098 and 0.0126 equivalent charges per A(2). These results represent the first description of the effects of surface charge screening on a TRP channel and provide a straightforward explanation for the known effects of extracellular Ca(2+) on cold-sensitive neurons.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.