Evidence map›Paper›PMID 19948652›Full record

ArticleThe Journal of physiology2010

Calcium activity in different classes of myenteric neurons underlying the migrating motor complex in the murine colon.

Peter O Bayguinov, Grant W Hennig, Terence K Smith

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
4.3field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 49 citations in OpenAlex.

  1. Article
  2. Imaging the enteric nervous system.Frontiers in neuroanatomy · 2025
    Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Optical Tools to Investigate Cellular Activity in the Intestinal Wall.Journal of neurogastroenterology and motility · 2015
    Article
  16. In situ Ca2+ imaging of the enteric nervous system.Journal of visualized experiments : JoVE · 2015
    Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Peter O BayguinovDepartment of Physiology and Cell Biology, Anderson Medical Sciences Building/352, University of Nevada School of Medicine, Reno, NV 89557, USA.
Grant W Hennig
Terence K Smith
University of Nevada, Reno · US

Funding

STRETCH DEPENDENT POTASSIUM CHANNEL REGULATION IN OVERACTIVE BLADDERP20RR018751 · NCRR · UNIVERSITY OF NEVADA RENO · PI SANDERS, KENTON M · 2003 to 2011
$17.3M
Relex Pathways Controlling Intestinal MotilityR01DK045713 · NIDDK · UNIVERSITY OF NEVADA RENO · PI SMITH, TERENCE KEITH · 1999 to 2012
$3.1M
NCRR NIH HHS P20 RR018751NCRR NIH HHS P20 RR-18751NIDDK NIH HHS R01 DK045713NIDDK NIH HHS R01 DK45713
6 · The paper itself

Abstract

The spontaneous colonic migrating motor complex (CMMC) is a cyclical contractile and electrical event that is the primary motor pattern underlying fecal pellet propulsion along the murine colon. We have combined Ca(2+) imaging with immunohistochemistry to determine the role of different classes of myenteric neurons during the CMMC. Between CMMCs, myenteric neurons usually displayed ongoing but uncoordinated activity. Stroking the mucosa at the oral or anal end of the colon resulted in a CMMC (latency: 6 to 10 s; duration: 28 s) that consisted of prolonged increases in activity in many myenteric neurons that was correlated to Ca(2+) transients in and displacement of the muscle. These neurons were likely excitatory motor neurons. Activity in individual neurons during the CMMC was similar regardless of whether the CMMC occurred spontaneously or was evoked by anal or oral mucosal stimulation. This suggests that convergent interneuronal pathways exist which generate CMMCs. Interestingly, Ca(2+) transients in a subset of NOS +ve neurons were substantially reduced during the CMMC. These neurons are likely to be inhibitory motor neurons that reduce their activity during a complex (disinhibition) to allow full excitation of the muscle. Local stimulation of the mucosa evoked synchronized Ca(2+) transients in Dogiel Type II (mitotracker/calbindin-positive) neurons after a short delay (1-2 s), indicating they were the sensory neurons underlying the CMMC. These local responses were observed in hexamethonium, but were blocked by ondansetron (5-HT(3) antagonist), suggesting Dogiel Type II neurons were activated by 5-HT release from enterochromaffin cells in the mucosa. In fact, removal of the mucosa yielded no spontaneous CMMCs, although many neurons (NOS +ve and NOS ve) exhibited ongoing activity, including Dogiel Type II neurons. These results suggest that spontaneous or evoked 5-HT release from the mucosa is necessary for the activation of Dogiel Type II neurons that generate CMMCs.

Indexed as

AnimalsCalciumColonFemaleGastrointestinal MotilityIntestinal MucosaMaleMiceMice, Inbred C57BLModels, AnimalMotor NeuronsMyenteric PlexusMyoelectric Complex, MigratingNitric Oxide SynthaseSerotoninSynaptic TransmissionCalciumNitric Oxide SynthaseSerotonin

Identifiers

PMID19948652
PMCPMC2825607
OpenAlexW1547991634

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.